Construct: ORF TRCN0000480424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003207.1_s317c1
- Derived from:
- ccsbBroadEn_04767
- DNA Barcode:
- TCTGGAGATGGACTTTCATACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOCS4 (122809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480424
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122809 | SOCS4 | suppressor of cytokine sign... | NM_080867.3 | 100% | 100% | |
| 2 | human | 122809 | SOCS4 | suppressor of cytokine sign... | NM_199421.2 | 100% | 100% | |
| 3 | human | 122809 | SOCS4 | suppressor of cytokine sign... | XM_011536425.1 | 100% | 100% | |
| 4 | human | 122809 | SOCS4 | suppressor of cytokine sign... | XM_011536426.1 | 100% | 100% | |
| 5 | mouse | 67296 | Socs4 | suppressor of cytokine sign... | NM_080843.2 | 85.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaaaataat gaaaatatta gtaaaaatgt agatgtaagg cccaaaacta 121 gtcggagcag aagtgccgac agaaaagacg gttatgtgtg gagtggaaag aagttatctt 181 ggtcaaaaaa gagtgagagt tattcagatg ctgagacagt gaatggtata gagaaaaccg 241 aagtgtcttt aaggaaccaa gaaaggaagc acagctgttc atccattgag ttggacttag 301 atcattcctg tgggcatcga tttttaggcc gatctcttaa acagaaactg caagatgccg 361 tggggcagtg ttttccaata aagaattgta gtagtcggca ctcttcaggg cttccgtcta 421 aaaggaaaat tcatatcagt gaactcatgt tagataagtg tcctttccca cctcgatcag 481 atttagcctt taggtggcat tttattaaac gacacactgc tcctataaat tccaaatcag 541 atgaatgggt aagcacagac ttgtctcaga ctgaattgag ggatggtcag ctaaaacgaa 601 gaaatatgga agaaaatata aactgtttct cacataccaa tgttcagccc tgtgtcataa 661 ccaccgacaa tgctttgtgt agagaaggtc ctatgactgg ctctgtgatg aacctggttt 721 caaataacag tatagaagat agtgatatgg attccgatga tgaaattcta acactttgca 781 caagttccag aaaaagaaac aaacccaaat gggatttgga tgatgaaatc ctgcagttgg 841 aaacacctcc taaataccac acgcagattg attatgtcca ctgtcttgta ccagacctcc 901 ttcagatcaa taacaaccca tgttactggG GAGTGATGGA TAAATACGCA GCCGAAGCAC 961 TACTGGAAGG AAAACCAGAG GGTACCTTTT TACTTCGAGA CTCAGCACAG GAAGACTATT 1021 TATTCTCTGT TAGTTTTAGA CGCTATAGTC GTTCTCTTCA TGCTAGAATT GAACAGTGGA 1081 ATCACAACTT TAGCTTTGAT GCACATGACC CCTGTGTCTT CCATTCTCCT GACATTACTG 1141 GGCTCCTAGA ACATTATAAG GACCCAAGCG CCTGTATGTT CTTTGAACCA CTTCTATCCA 1201 CTCCCTTAAT TCGGACTTTC CCTTTTTCCC TGCAGCATAT ATGCAGAACA GTTATTTGTA 1261 ACTGTACAAC TTATGATGGC ATCGATGCCC TTCCAATTCC TTCTTCTATG AAATTATATC 1321 TGAAGGAATA TCATTATAAA TCAAAAGTTA GAGTACTCAG GATTGATGCA CCAGAACAGC 1381 AATGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTGGAGAT GGACTTTCAT ACAAAACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt