Construct: ORF TRCN0000480425
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002704.1_s317c1
- Derived from:
- ccsbBroadEn_05777
- DNA Barcode:
- AAGTATATATCCCGTTTTGCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH1C (126)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480425
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NM_000669.5 | 99.9% | 100% | 453A>G |
| 2 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_000668.6 | 95.7% | 94.4% | (many diffs) |
| 3 | human | 124 | ADH1A | alcohol dehydrogenase 1A (c... | NM_000667.4 | 93.7% | 92.8% | (many diffs) |
| 4 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_001286650.2 | 85.4% | 84.5% | (many diffs) |
| 5 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NR_133005.2 | 74.2% | (many diffs) | |
| 6 | mouse | 11522 | Adh1 | alcohol dehydrogenase 1 (cl... | NM_007409.2 | 82.4% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cacagcagga aaagtaatca aatgcaaagc agctgtgcta tgggagttaa 121 agaaaccctt ttccattgag gaggtagagg ttgcacctcc taaggctcat gaagttcgca 181 ttaagatggt ggctgcagga atctgtcgtt cagatgagca tgtggttagt ggcaacctgg 241 tgacccccct tcctgtgatt ttaggccatg aggcagccgg catcgtggaa agtgttggag 301 aaggggtgac tacagtcaaa ccaggtgata aagtcatccc gctctttact cctcagtgtg 361 gaaaatgcag aatttgtaaa aacccagaaa gcaactactg cttgaaaaat gatctaggca 421 atcctcgggg gaccctgcag gatggcacca ggaggttcac ctgcagcggg aagcccatcc 481 accacttcgt cggcgtcagc accttctccc agtacacggt ggtggatgag aatgcagtgg 541 ccaaaattga tgcagcctcg cccctggaga aagtctgcct cattggctgt ggattttcga 601 ctggttatgg gtctgcagtc aaagttgcca aggtcacccc agggtctacc tgtgctgtgt 661 ttggcctggg aggggtcggc ctatctgttg ttatgggctg taaagcagct ggagcagcca 721 gaatcattgc tgtggacatc aacaaggaca aatttgcaaa ggctaaagag ttgggtgcca 781 ctgaatgcat caaccctcaa gactaCAAGA AACCCATTCA GGAAGTGCTA AAGGAAATGA 841 CTGATGGAGG TGTGGATTTT TCGTTTGAAG TCATCGGTCG GCTTGACACC ATGATGGCTT 901 CCCTGTTATG TTGTCATGAG GCATGTGGCA CAAGTGTCAT TGTAGGGGTA CCTCCTGATT 961 CCCAGAACCT CTCAATAAAC CCTATGCTGC TACTGACTGG ACGCACGTGG AAAGGAGCTA 1021 TTTTTGGAGG CTTTAAGAGT AAAGAATCTG TCCCCAAACT TGTGGCTGAC TTTATGGCTA 1081 AGAAGTTTTC ACTGGATGCA TTAATAACAA ATATTTTACC TTTTGAAAAA ATAAATGAAG 1141 GATTTGACCT GCTTCGCTCT GGAAAGAGTA TCCGTACCGT CCTGACGTTT TGCCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAAAGT ATATATCCCG TTTTGCATTT ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt