Construct: ORF TRCN0000480441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010364.1_s317c1
- Derived from:
- ccsbBroadEn_08600
- DNA Barcode:
- ATCCGCTTCCAATAACCACGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIZ (55857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55857 | KIZ | kizuna centrosomal protein | NM_018474.6 | 99.9% | 99.7% | 417C>G;707T>C |
| 2 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001352434.2 | 97.2% | 97% | 417C>G;707T>C;1390_1391ins54 |
| 3 | human | 55857 | KIZ | kizuna centrosomal protein | XM_011529296.3 | 95.1% | 92.2% | (many diffs) |
| 4 | human | 55857 | KIZ | kizuna centrosomal protein | XM_011529297.3 | 92.5% | 89.6% | (many diffs) |
| 5 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001276389.2 | 85.3% | 86.2% | (many diffs) |
| 6 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001163022.3 | 84.4% | 84.1% | (many diffs) |
| 7 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001352436.2 | 82.9% | 82.7% | 0_1ins342;75C>G;365T>C |
| 8 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001352435.2 | 81.8% | 81.4% | (many diffs) |
| 9 | human | 55857 | KIZ | kizuna centrosomal protein | XM_011529299.3 | 80.1% | 77.1% | (many diffs) |
| 10 | human | 55857 | KIZ | kizuna centrosomal protein | NM_001163023.3 | 80% | 79.6% | (many diffs) |
| 11 | human | 55857 | KIZ | kizuna centrosomal protein | XM_017027951.2 | 78.6% | 75.8% | (many diffs) |
| 12 | human | 55857 | KIZ | kizuna centrosomal protein | XM_017027952.2 | 77.5% | 74.6% | (many diffs) |
| 13 | human | 55857 | KIZ | kizuna centrosomal protein | XR_937105.3 | 77.3% | (many diffs) | |
| 14 | human | 55857 | KIZ | kizuna centrosomal protein | XR_001754334.2 | 72.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2088
- ORF length:
- 2019
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccggacc ctcgcatcgg ccgtgcccct gtcgagtccc gactactacg 121 agaggctggg ccaactccag cacgggctgc gggacagtga aaagaagaga ttggacctgg 181 aaaagaaact ttatgaatat aatcagtctg atacatgcag agttaagctg aaatatgtaa 241 aactaaagaa ttatctgaag gaaatatgtg aatctgaaaa gaaggctcat actcgaaacc 301 aagaatattt aaagcgattt gagcgtgtcc aagctcatgt tgtacacttc accacaaata 361 cagagaagct tcaaaaactg aagctcgaat atgagactca aattaagaag atgctatgct 421 caaaagatag cctgggacta aaagaggaac tgacagatga agacagagaa aaggttgcag 481 tgcaggaggg gattaactca ggaacagcca tgtcaagagg attgtatcaa ccagcaacaa 541 tctttatggg ccgccaaatg tcagccatct taagcatgag agatttcagt acagagcaca 601 aatctcccca gcccacaaag aacttttcaa ttcctgaccc acattcacac cgacagacag 661 cccagagcag taatgtgaca gacagctgtg tagtacaaac tagtaatgac acacagtgct 721 taaataagtc tgacaacata gatggaaagg catctcttca gattggtgag aaaacgccag 781 tcacagccag tgtattgtct gaggaggaac aaactcattg cttggagata ggaagtaaca 841 cacgtcatgg caagagtaat ttatctgaag gcaaaaagtc tgctgaactc aattccccgt 901 tacgggaaag attaagtcca gagaacagaa ccactgattt aaagtgtgac agttccagcg 961 gatcagaggg agaaatactg acacgggaac atattgaagt tgaggaaaaa agagccagcc 1021 cgccagtctc tccgatacca gtttcagaat actgtgaatc tgaaaataag tggtctcaag 1081 agaagcattc tccttgggaa ggtgtttcag atcatcttgc tcacagggaa ccaaagtcac 1141 aaaagccctt cagaaaaatg caggaagagg aggaggaaag ttggagcacc agcagtgacc 1201 ttaccatttc aataagtgaa gatgatctga ttttagagag cccagaacca cagccaaatc 1261 caggtggcaa gatggaggga gaagatggaa tagaggcctt aaaattaatc catgctgagc 1321 aagaaagagt tgccctatcc actgaaaaaa attgtatttt gcaaacccta agctctcctg 1381 attcagaaaa ggaatcctcc actaacgcac caacaagaga acctggacaa acaccagact 1441 cagacgtacc gagggcacag gtgggtcagc atgttgccac cttgaaagaa catgataatt 1501 ctgtcaaaga agaggcaaca gcattattga gaaaagccct tacagaagag tgtggccgta 1561 ggtcagctat tcacagtagt gaatcatctt gcagcttgcc atctattctg aatgacaata 1621 gtggaataaa ggaagccaaa cctgctgtat ggctcaacag tgttcctaca agggaacaag 1681 aagtttcaag tggctgtgga gacaagagca agaaagaaaa tgtggctgca gatatcccaa 1741 tcacagaaac agaagcctat cagttgctga agaaggccac ccttcaggat aatacaaatc 1801 aaactGAAAA CAGGTTTCAA AAGACAGATG CTTCTGTGTC ACACTTGTCA GGTTTGAATA 1861 TTGGCAGCGG TGCATTCGAG ACAAAGACAG CTAACAAAAT TGCTTCGGAA GCTAGTTTTT 1921 CATCTAGTGA AGGAAGTCCT TTGTCAAGGC ATGAAAACAA AAAGAAACCC GTGATCAATT 1981 TAAAATCTAA TGCCCTCTGG GATGAGTCTG ATGACAGTAA CTCAGAAATT GAGGCTGCTT 2041 TACGCCCCAG AAACCATAAC ACCGATGATT CTGATGATTT TTATGACTTG CCAACTTTCT 2101 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2161 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2221 GTGGAAAGGA CGAATCCGCT TCCAATAACC ACGCATCACG CGTTAAGTCg acaatcaacc 2281 tctggattac aaaatttgtg aaagatt