Construct: ORF TRCN0000480447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009002.1_s317c1
- Derived from:
- ccsbBroadEn_11410
- DNA Barcode:
- AGACCCGTCTGTCAAACGTTTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOCK4 (9732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_006716189.2 | 56.6% | 55.3% | (many diffs) |
| 2 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012825.1 | 56% | 54.3% | (many diffs) |
| 3 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | NM_014705.4 | 55.7% | 54.5% | (many diffs) |
| 4 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012824.1 | 55.7% | 54% | (many diffs) |
| 5 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | NM_001363540.2 | 55.5% | 54.2% | (many diffs) |
| 6 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012823.1 | 55.5% | 53.8% | (many diffs) |
| 7 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012821.1 | 54.9% | 53.2% | (many diffs) |
| 8 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012822.1 | 54.9% | 53.4% | (many diffs) |
| 9 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012820.1 | 54.6% | 53% | (many diffs) |
| 10 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_017012819.1 | 54.4% | 52.8% | (many diffs) |
| 11 | human | 9732 | DOCK4 | dedicator of cytokinesis 4 | XM_024447006.1 | 51.6% | 50.4% | (many diffs) |
| 12 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_017315054.1 | 51.8% | 54.9% | (many diffs) |
| 13 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515090.2 | 51.3% | 54.4% | (many diffs) |
| 14 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515089.2 | 51.2% | 54.4% | (many diffs) |
| 15 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515088.2 | 50.7% | 53.8% | (many diffs) |
| 16 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515087.2 | 50.5% | 53.6% | (many diffs) |
| 17 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515086.2 | 50.4% | 53.6% | (many diffs) |
| 18 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | NM_172803.2 | 50.3% | 53.4% | (many diffs) |
| 19 | mouse | 238130 | Dock4 | dedicator of cytokinesis 4 | XM_006515085.2 | 50.3% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3387
- ORF length:
- 3318
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggatacct acggagcacg agaaatacgg cgtggttatt gccagtttcc 121 gaggaaccgt tccatatggc ctgtcattgg aaattggaga tacagttcag atcctggaga 181 agtgtgatgg ctggtacaga ggatttgcct taaaaaaccc aaatatcaag ggtatatttc 241 cttccagcta cgttcacttg aaaaatgcct gtgtaaagaa caaaggacaa tttgaaatgg 301 ttattcccac tgaagactct gttatcacag aaatgacatc aacattaaga gactggggaa 361 ccatgtggaa acaactctat gtgcgtaatg aaggcgatct cttccaccgg ctgtggcaca 421 tcatgaatga aatcctggac ctgaggcggc aggtgctggt gggccacctc acccacgacc 481 ggatgaagga cgtgaagcgc cacattactg cccggcttga ctggggcaat gaacaactgg 541 gactggacct ggtgcctagg aaagagtacg caatggtgga tccggaagac atcagcatta 601 ctgagctcta ccgattgatg gaacatcgac atcggaagaa agacaccccg gtgcaggcca 661 gcagtcacca cctctttgtc cagatgaaga gcctcatgtg ttccaacctg ggagaggagc 721 tggaggtcat cttctcactc tttgacagta aagagaaccg gccaatcagt gagagatttt 781 tcttgaggct gaatagaaac gggcttccca aagcccctga taaaccggaa cgacattgct 841 ccctctttgt ggatttgggc agcagtgagc taagaaagga catttatatc accgtgcaca 901 ttatccgaat cggtcgaatg ggagcaggag aaaaaaagaa tgcctgtagt gtccagtacc 961 gacgaccctt tggctgtgca gttcttagca tcgctgacct gctaacagga gagacaaagg 1021 atgacctcat tctgaaagta tacatgtgta acacagagag tgagtggtac caaatccatg 1081 agaacatcat caaaaagctg aatgcacgtt ataacttgac tggctccaat gcaggtttag 1141 cagtttcctt acagctattg cacggagaca ttgaacaaat cagaagggaa tattcatcag 1201 tattttctca tggagtatcc ataacaagga agctgggatt ttcaaatatt attatgcctg 1261 gtgaaatgag gaatgattta tatatcacta ttgaaagggg agaatttgag aaaggaggga 1321 agagcgtggc cagaaatgtg gaagttacga tgttcattgt agacagtagt ggccaaaccc 1381 tgaaggattt tatctccttc ggctctgggg agccaccagc cagtgagtac cactcctttg 1441 tgctttacca taacaacagt cccaggtggt ctgaactgct gaaacttccc attcctgtgg 1501 ataaattccg gggtgcacac atccgcttcg agtttcggca ttgttccaca aaggagaaag 1561 gagagaagaa gttgtttggg ttttcttttg tccctctgat gcaagaagat ggtaggactc 1621 ttccagatgg cactcatgag ctcatcgtgc ataagtgtga agaaaacaca aatcttcagg 1681 atactacccg ctacctcaaa cttccctttt ccaagggcat tttccttggg aataataatc 1741 aagccatgaa ggccacaaag gagtcctttt gtattacatc ttttctctgt tccacaaaac 1801 tcacacaaaa tggtgatatg cttgatcttt tgaaatggag aacccaccca gacaagatca 1861 ctggctgtct ctctaaatta aaagaaattg atggctcaga gatagtaaag tttctgcagg 1921 atacactgga taccttattt ggaattttag atgaaaattc ccaaaaatat gggtctaaag 1981 tgtttgattc tttggttcac ataataaatt tgctgcaaga tagcaaattt catcatttta 2041 aacctgtaat ggacacttac attgagagtc attttgctgg ggcacttgca tacagagatc 2101 tcatcaaagt gctcaaatgg tacgtggacc ggatcacaga agcagagcgg caagagcata 2161 tccaggaggt gctgaaggca caagaataca tttttaagta tatagttcaa tctcgaaggc 2221 tgttttccct tgccactggt gggcaaaacg aagaggagtt ccgctgctgc attcaggagc 2281 ttctcatgtc agtccgtttc tttctttcgc aagagagcaa agggtctgga gcattatctc 2341 agtcacaggc tgtgtttctg agctctttcc ctgccgtgta ctcagaactg ttgaagctct 2401 ttgatgtccg ggaagtagcc aacttggtcc aggacaccct gggcagtctg ccgaccatcc 2461 tgcatgtgga tgattccctg caggccatca aactgcagtg cattggcaaa accgtggaaa 2521 gccagcttta taccaaccca gattcccgat acattcttct gcctgtcgtg ttacatcacc 2581 tccacattca cttgcaagaa cagaaggacc tgatcatgtg tgcacgtatc cttagcaacg 2641 tattttgtct tatcaagaaa aatagctcag aaaaatctgt gctggaggaa atagatgtga 2701 tagtggccag cttgctggat attctgctga ggaccatatt ggagatcacc agccgacctc 2761 agccatccag ctcagcaatg cggttccagt tccaggatgt cactggggag tttgttgctt 2821 gtctcctgtc cctattacga caaatgacag atagacatta tcaacagctt cttgatagtt 2881 ttaatacaaa ggaagaacta agggatttcc tgctgcagat atttactgtg ttccgaatat 2941 tgatacgccc ggagatgttt ccaaaggact ggactgttat gcgcttggtt gctaacaatg 3001 ttattattac aacagttcta tacctctcag atgcacttcg taagaacttc ttaaatgaaa 3061 actttgatta taagatctgg gattcctact tttacctcgc agtcattttt ataaaccagt 3121 tgtgtctgca gttggagatg ttcacaCCTT CCAAGAAGAA AAAGGTGTTA GAAAAGTATG 3181 GTGACATGCG GGTAACAATG GGTTGTGAAA TTTTCAGCAT GTGGCAAAAC CTAGGAGAGC 3241 ACAAGCTTCA TTTTATCCCT GCCCTGAGGC AGGAGAATCG CTTGAACCTG GGAGGAGGAG 3301 GTTGCAGTGA GCCGAGAGCA TGTCACTGCA CTCCAGCCTG GGCAACAAAG CGAGACTGTG 3361 TCTCAAAGAA AAAAGAAAAA AAGACATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGACCCGT 3541 CTGTCAAACG TTTCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt