Construct: ORF TRCN0000480461
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014986.1_s317c1
- Derived from:
- ccsbBroadEn_04247
- DNA Barcode:
- TGGTAACACGCATGTACCCTTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITM2C (81618)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480461
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81618 | ITM2C | integral membrane protein 2C | NM_001012514.3 | 100% | 100% | |
| 2 | human | 81618 | ITM2C | integral membrane protein 2C | NM_001287240.2 | 88.1% | 78.2% | (many diffs) |
| 3 | human | 81618 | ITM2C | integral membrane protein 2C | NM_001287241.2 | 82.3% | 82.3% | 120_260del |
| 4 | human | 81618 | ITM2C | integral membrane protein 2C | NM_030926.6 | 82.3% | 82.3% | 120_260del |
| 5 | human | 81618 | ITM2C | integral membrane protein 2C | NM_001012516.2 | 68.5% | 68.5% | 120_260del;449_450ins111 |
| 6 | mouse | 64294 | Itm2c | integral membrane protein 2C | NM_022417.3 | 72.4% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaagattagc ttccagcccg ccgtggctgg catcaagggc gacaaggctg 121 acaaggcgtc ggcgtcggcc cctgcgccgg cctcggccac cgagatcctg ctgacgccgg 181 ctaggctggc ccgagataac ttcttccgct gtggtgtgct gtatgaggac tccctgtcct 241 cccaggtccg gactcagatg gagctggaag aggatgtgaa aatctacctc gacgagaact 301 acgagcgcaT CAACGTGCCT GTGCCCCAGT TTGGCGGCGG TGACCCTGCA GACATCATCC 361 ATGACTTCCA GCGGGGTCTG ACTGCGTACC ATGATATCTC CCTGGACAAG TGCTATGTCA 421 TCGAACTCAA CACCACCATT GTGCTGCCCC CTCGCAACTT CTGGGAGCTC CTCATGAACG 481 TGAAGAGGGG GACCTACCTG CCGCAGACGT ACATCATCCA GGAGGAGATG GTGGTCACGG 541 AGCATGTCAG TGACAAGGAG GCCCTGGGGT CCTTCATCTA CCACCTGTGC AACGGGAAAG 601 ACACCTACCG GCTCCGGCGC CGGGCAACGC GGAGGCGGAT CAACAAGCGT GGGGCCAAGA 661 ACTGCAATGC CATCCGCCAC TTCGAGAACA CCTTCGTGGT GGAGACGCTC ATCTGCGGGG 721 TGGTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGGTAACAC GCATGTACCC TTGCCACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt