Transcript: Human NM_001012514.3

Homo sapiens integral membrane protein 2C (ITM2C), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
ITM2C (81618)
Length:
1896
CDS:
85..747

Additional Resources:

NCBI RefSeq record:
NM_001012514.3
NBCI Gene record:
ITM2C (81618)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001012514.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156662 GCTTGTACTTTGGACGCGTTT pLKO.1 832 3UTR 100% 4.050 5.670 N ITM2C n/a
2 TRCN0000154864 GACAAGTGCTATGTCATCGAA pLKO.1 424 CDS 100% 3.000 4.200 N ITM2C n/a
3 TRCN0000156941 GATAACTTCTTCCGCTGTGGT pLKO.1 214 CDS 100% 2.640 3.696 N ITM2C n/a
4 TRCN0000157813 CGAACTCAACACCACCATTGT pLKO.1 441 CDS 100% 4.950 3.960 N ITM2C n/a
5 TRCN0000156586 GAGCTGGAAGAGGATGTGAAA pLKO.1 280 CDS 100% 4.950 3.465 N ITM2C n/a
6 TRCN0000154865 GCTATGTCATCGAACTCAACA pLKO.1 431 CDS 100% 4.950 3.465 N ITM2C n/a
7 TRCN0000156771 GCAGACATCATCCATGACTTC pLKO.1 367 CDS 100% 4.050 2.835 N ITM2C n/a
8 TRCN0000158284 CGAGATAACTTCTTCCGCTGT pLKO.1 211 CDS 100% 2.160 1.512 N ITM2C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001012514.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04247 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04247 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480461 TGGTAACACGCATGTACCCTTGCC pLX_317 62.7% 100% 100% V5 n/a
4 ccsbBroadEn_09078 pDONR223 100% 68.4% 68.5% None 119_120ins141;309_419del;657G>A n/a
5 TRCN0000480102 ATGGCGTAAACACAGTCAACTGGT pLX_317 52.3% 68.4% 68.5% V5 119_120ins141;309_419del;657G>A n/a
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