Construct: ORF TRCN0000480463
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014565.1_s317c1
- Derived from:
- ccsbBroadEn_10026
- DNA Barcode:
- CCTCAACATTAAGACCTAACATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF517 (340385)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480463
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340385 | ZNF517 | zinc finger protein 517 | NM_213605.2 | 99.8% | 99.5% | 541G>A;1046T>C |
| 2 | human | 340385 | ZNF517 | zinc finger protein 517 | XM_011517014.3 | 99.8% | 99.5% | 541G>A;1046T>C |
| 3 | human | 340385 | ZNF517 | zinc finger protein 517 | XM_011517015.3 | 99.8% | 99.5% | 541G>A;1046T>C |
| 4 | human | 340385 | ZNF517 | zinc finger protein 517 | NM_001317936.1 | 98.4% | 98.1% | 158_159ins21;520G>A;1025T>C |
| 5 | human | 340385 | ZNF517 | zinc finger protein 517 | XM_011517017.3 | 92.8% | 87.8% | (many diffs) |
| 6 | human | 340385 | ZNF517 | zinc finger protein 517 | XM_017013384.2 | 80.7% | 80.4% | 0_1ins282;259G>A;764T>C |
| 7 | human | 340385 | ZNF517 | zinc finger protein 517 | NR_134284.1 | 53.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgatggca ctcccgatgc ctggacctca ggaggcggtt gtgttcgagg 121 atgtggctgt gtacttcaca aggatagagt ggagttgcct ggcccccgac cagcaggcac 181 tctacaggga cgtgatgctg gagaactatg ggaacctggc ctcactaggc tttcttgttg 241 ccaaaccagc actgatctcc ctattggagc aaggagagga gccgggggcc ttgattctgc 301 aggtggctga acagagcgtg gccaaagcca gcctgtgcac agattccagg atggaggctg 361 ggatcatgga gtctcctctg cagagaaagc tctccaggca ggcaggactg ccgggcaccg 421 tgtgggggtg cctcccctgg gggcaccctg tgggggggca ccctgcacca ccccacccgc 481 atggcggtcc tgaggacggg tcagataaac ccacccaccc ccgggctcgg gagcacagcg 541 cctccccaag ggttctgcag gaagacctgg gccggcctgt ggggagctca gccccccgct 601 acaggtgcat gtgcggcaag gcgttcagat acaactcgct gcttctcagg caccagatca 661 tccacaccgg cgccaagccc ttccagtgca cagagtgcgg gaaggccttc aagcaaagct 721 ccatcctgct gcggcaccag ctgatccaca ctgaggagaa gccgttccag tgcggcgagt 781 gcgggaaggc cttccggcag agcacgcagc tggctgccca ccaccgcgtc cacacccgcg 841 agcggcccta cgcatgcggc gagtgcggca aggccttcag ccgcagctcc cggctgctgc 901 agcaccagaa gttccacacc ggggagaagc ccttcgcgtg cacagagtgc ggcaaggcgt 961 tctgccgcag gttcaccctc aacgagcacg gccgcatcca cagcggggag cggccctacc 1021 ggtgcctgcg gtgtgggcag cgcttcatcc gagggtcctc gctcctgaag caccaccggc 1081 tgcacgcgca ggagggtgcc caggacggcg gcgcggggca gggcgccctg cTCGGAGCTG 1141 CGCAGAGGCC CCAGGCGGGG GACCCGCCCC ACGAGTGCCC GGTGTGCGGG AGGCCGTTCC 1201 GACACAACTC CCTGCTGCTG CTGCACCTGC GCCTACACAC GGGCGAGAAG CCGTTCGAGT 1261 GCGCGGAGTG CGGCAAGGCC TTCGGTCGCA AGTCCAACCT CACTCTGCAC CAGAAGATCC 1321 ACACCAAGGA GAAGCCCTTC GCGTGCACCG AGTGCGGCAA GGCGTTCCGC AGGAGCTACA 1381 CGCTGAACGA GCACTACCGG CTCCACAGCG GCGAGAGGCC ATACCGGTGC CGCGCCTGCG 1441 GGAGGGCCTG CAGCCGGCTG TCCACCCTCA TCCAGCACCA GAAGGTGCAC GGCCGCGAGC 1501 CCGGGGAGGA CACAGAGGGC AGGCGGGCGC CCTGTTGGGC TTCCTTGCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA CCTCAACATT AAGACCTAAC ATTCACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt