Transcript: Human XM_011517014.3

PREDICTED: Homo sapiens zinc finger protein 517 (ZNF517), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF517 (340385)
Length:
6867
CDS:
647..2125

Additional Resources:

NCBI RefSeq record:
XM_011517014.3
NBCI Gene record:
ZNF517 (340385)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011517014.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000107750 AGGTGCTGTAAAGCCGACTAA pLKO.1 2551 3UTR 100% 4.950 6.930 N ZNF517 n/a
2 TRCN0000107753 GCTGATCCACACTGAGGAGAA pLKO.1 1318 CDS 100% 4.050 2.835 N ZNF517 n/a
3 TRCN0000107751 GCTGCTTCTCAGGCACCAGAT pLKO.1 1216 CDS 100% 1.350 0.810 N ZNF517 n/a
4 TRCN0000107754 GCGCCTACACACGGGCGAGAA pLKO.1 1807 CDS 100% 0.000 0.000 N ZNF517 n/a
5 TRCN0000138140 GATTGAGACCATCCTGGCTAA pLKO.1 6839 3UTR 100% 4.050 2.025 Y LOC441087 n/a
6 TRCN0000107752 GCACCAGAAGATCCACACCAA pLKO.1 1885 CDS 100% 2.640 1.320 Y ZNF517 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011517014.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10026 pDONR223 100% 99.8% 99.5% None 541G>A;1046T>C n/a
2 ccsbBroad304_10026 pLX_304 0% 99.8% 99.5% V5 541G>A;1046T>C n/a
3 TRCN0000480463 CCTCAACATTAAGACCTAACATTC pLX_317 28.2% 99.8% 99.5% V5 541G>A;1046T>C n/a
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