Construct: ORF TRCN0000480464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014316.1_s317c1
- Derived from:
- ccsbBroadEn_08029
- DNA Barcode:
- TATATAACCTGGTAATACAATGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRPK3 (26576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170760.2 | 99.9% | 100% | 1011A>G |
| 2 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_014370.4 | 99.7% | 99.8% | 774_776delGCA;1014A>G |
| 3 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170761.1 | 94.1% | 94.1% | 948_949ins99 |
| 4 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | NM_019684.2 | 85.8% | 94.3% | (many diffs) |
| 5 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_006528172.3 | 82.1% | 90.3% | (many diffs) |
| 6 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_017318579.1 | 38% | 40.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1767
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgccagc acgggcggtg gtggggacag cggcggcagc ggcggcagta 121 gcagcagctc acaggcctcc tgcgggcccg agtcctcggg ctccgaacta gccctggcca 181 caccggtgcc tcagatgctg cagggccttc tgggctccga cgacgaggaa caggaagacc 241 ccaaagacta ctgcaagggc ggctaccacc ctgtgaagat cggcgacgtg ttcaatgggc 301 ggtaccacgt ggtgcgcaaa ctgggctggg gccacttctc caccgtctgg ctctgctggg 361 acatccagcg caagcgcttt gtggccctca aagtggtgaa gagtgcgggg cattacacgg 421 agacagctgt ggatgagatc aagctcctga aatgtgtccg ggacagcgac cccagtgacc 481 ccaaaagaga gaccattgtc cagctcattg atgacttcag gatctcagga gtcaatggag 541 tccatgtgtg catggtgctg gaggtgctgg gccaccagct cctcaaatgg atcatcaagt 601 ccaactacca gggcctgccc gtgccctgcg tgaagagcat cgtgaggcag gtgctgcacg 661 gcctggacta cctccacacc aagtgcaaga tcatccacac ggacatcaag cccgagaaca 721 tcttgctgtg tgtgggggac gcttacatca ggcgcctggc tgccgaggcc acggagtggc 781 aacaggcagg ggcgccgccc ccctcccgct ccatagtcag cactgccccc caggaggtct 841 tgaccggtaa gctgtccaaa aacaagagga agaagatgag gcgcaaacgg aaacagcaga 901 agcggctgct ggaggagcgg ctgcgggacc tgcagaggct ggaggccatg gaggctgcca 961 cccaggctga ggactctggc ttgagactag acgggggcag cggctccaca tcctcttcag 1021 gctgtcaccc cgggggcgcc agagcaggtc cctccccagc ctcttcctcc cccgccccgg 1081 ggggcggccg tagcctcagc gcgggctcac agacctcagg cttctccggc tccctcttct 1141 ctcctgcctc ctgctccatc ctctccggct cgtccaatca gcgagagacc gggggcctcc 1201 tgtcgcctag cacaccattc ggtgcctcga acctcctggt gaaccccctg gagccccaaa 1261 atgcagataa gatcaagatc aagatcgcag acctgggcaa cgcctgctgg gtgcacaagc 1321 acttcacgga agacatccag actcggcagt accgggccgt cgaggtgctg atcggcgccg 1381 aatacggccc cccggcagac atctggagca cagcctgcat ggccTTCGAG CTGGCCACTG 1441 GTGACTACCT GTTCGAGCCG CATTCTGGAG AAGACTACAG TCGTGATGAG GACCACATCG 1501 CTCACATAGT GGAGCTTCTG GGGGACATCC CCCCAGCCTT CGCCCTCTCA GGCCGCTATT 1561 CCCGGGAGTT CTTCAACCGG AGAGGAGAGC TGCGGCACAT CCACAATCTC AAGCACTGGG 1621 GCCTGTACGA GGTACTCATG GAAAAGTACG AGTGGCCCCT AGAGCAGGCC ACACAGTTCA 1681 GCGCCTTTCT GCTGCCCATG ATGGAGTACA TCCCCGAAAA GCGGGCCAGT GCCGCTGACT 1741 GCCTCCAGCA CCCCTGGCTC AACCCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1801 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1861 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATATAAC 1921 CTGGTAATAC AATGCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1981 aagatt