Transcript: Mouse XM_006528172.3

PREDICTED: Mus musculus serine/arginine-rich protein specific kinase 3 (Srpk3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Srpk3 (56504)
Length:
2389
CDS:
380..2152

Additional Resources:

NCBI RefSeq record:
XM_006528172.3
NBCI Gene record:
Srpk3 (56504)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006528172.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000368735 GGAGACCATTGTTCAACTTAT pLKO_005 868 CDS 100% 13.200 18.480 N Srpk3 n/a
2 TRCN0000361742 TTGCCCTCTCAGGCCGATATT pLKO_005 1923 CDS 100% 13.200 18.480 N Srpk3 n/a
3 TRCN0000025956 GCTCATATCGTGGAACTTCTA pLKO.1 1883 CDS 100% 4.950 6.930 N Srpk3 n/a
4 TRCN0000025954 CCCAGTGAAGATCGGTGATTT pLKO.1 649 CDS 100% 13.200 10.560 N Srpk3 n/a
5 TRCN0000368796 AGCACTTCACTGAGGATATTC pLKO_005 1701 CDS 100% 13.200 9.240 N Srpk3 n/a
6 TRCN0000025931 CCCTGTGTTAAGAGCATTGTT pLKO.1 1004 CDS 100% 5.625 3.938 N Srpk3 n/a
7 TRCN0000025892 CCTCCATACTAAGTGCAAGAT pLKO.1 1051 CDS 100% 4.950 3.465 N Srpk3 n/a
8 TRCN0000025925 GCTGTGGACAGTAGAGACTAT pLKO.1 2164 3UTR 100% 4.950 3.465 N Srpk3 n/a
9 TRCN0000218695 ATGAGATCAAGCTCCTGAAAT pLKO_005 813 CDS 100% 13.200 7.920 N SRPK3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006528172.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08029 pDONR223 100% 82.1% 90.3% None (many diffs) n/a
2 ccsbBroad304_08029 pLX_304 0% 82.1% 90.3% V5 (many diffs) n/a
3 TRCN0000480464 TATATAACCTGGTAATACAATGCC pLX_317 20.5% 82.1% 90.3% V5 (many diffs) n/a
4 TRCN0000488078 GCATTTTTACTGCACTATGGCCTT pLX_317 14.2% 82.1% 90.3% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489375 GGTTACAGCATCAAGTGAACATCA pLX_317 20.5% 82.1% 90.2% V5 (many diffs) n/a
6 ccsbBroadEn_15037 pDONR223 97.1% 80.7% 36.8% None (many diffs) n/a
7 ccsbBroad304_15037 pLX_304 0% 80.7% 36.8% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000472667 AACACTAAATACATGGCAACCTCA pLX_317 20.4% 80.7% 36.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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