Transcript: Human NM_014370.4

Homo sapiens SRSF protein kinase 3 (SRPK3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
SRPK3 (26576)
Length:
1960
CDS:
47..1750

Additional Resources:

NCBI RefSeq record:
NM_014370.4
NBCI Gene record:
SRPK3 (26576)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148156 CCTGCGTGAAGAGCATCGTG pXPR_003 AGG 573 34% 6 1.053 LOC124905227, SRPK3 SRPK3 77397
2 BRDN0001146140 TCGGCGACGTGTTCAATGGG pXPR_003 CGG 228 13% 3 0.7792 LOC124905227, SRPK3 SRPK3 77396
3 BRDN0001146524 GCTGATTGGACGAGCCGGAG pXPR_003 AGG 1100 65% 10 0.0789 LOC124905227, SRPK3 SRPK3 77395
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014370.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007067 GAGACCATTGTCCAGCTCATT pLKO.1 467 CDS 100% 4.950 6.930 N SRPK3 n/a
2 TRCN0000368372 GATGAGGCGCAAACGGAAACA pLKO_005 856 CDS 100% 4.950 6.930 N SRPK3 n/a
3 TRCN0000199585 GAGGTCTTGCAGACCGGTAAG pLKO.1 812 CDS 100% 2.000 2.800 N SRPK3 n/a
4 TRCN0000199552 CCTGTCGCCTAGCACACCATT pLKO.1 1180 CDS 100% 1.650 2.310 N SRPK3 n/a
5 TRCN0000364062 AGAGCGTGTTCTGCCTGAGAC pLKO_005 1880 3UTR 100% 1.350 1.890 N SRPK3 n/a
6 TRCN0000199923 GCTCGTCCAATCAGCGAGAGA pLKO.1 1149 CDS 100% 0.880 1.232 N SRPK3 n/a
7 TRCN0000199146 CGGGAGTTCTTCAACCGGAGA pLKO.1 1544 CDS 100% 0.720 1.008 N SRPK3 n/a
8 TRCN0000199786 GAGCGTGTTCTGCCTGAGACC pLKO.1 1881 3UTR 100% 0.000 0.000 N SRPK3 n/a
9 TRCN0000368373 TACCTGTTCGAGCCGCATTCT pLKO_005 1427 CDS 100% 0.000 0.000 N SRPK3 n/a
10 TRCN0000234325 CTCTCCGTGCCTTAAGGGAAA pLKO_005 1775 3UTR 100% 4.050 3.240 N SRPK3 n/a
11 TRCN0000218695 ATGAGATCAAGCTCCTGAAAT pLKO_005 411 CDS 100% 13.200 9.240 N SRPK3 n/a
12 TRCN0000364066 AGACCATTGTCCAGCTCATTG pLKO_005 468 CDS 100% 10.800 7.560 N SRPK3 n/a
13 TRCN0000234322 AGATCGGCGACGTGTTCAATG pLKO_005 255 CDS 100% 10.800 7.560 N SRPK3 n/a
14 TRCN0000234324 AGGACCACATCGCTCACATAG pLKO_005 1470 CDS 100% 10.800 7.560 N SRPK3 n/a
15 TRCN0000234323 TCAGGATCTCAGGAGTCAATG pLKO_005 495 CDS 100% 10.800 7.560 N SRPK3 n/a
16 TRCN0000364063 CTGCCCATGATGGAGTACATC pLKO_005 1673 CDS 100% 4.950 3.465 N SRPK3 n/a
17 TRCN0000199707 GAGGACCACATCGCTCACATA pLKO.1 1469 CDS 100% 4.950 3.465 N SRPK3 n/a
18 TRCN0000368316 GCGGCACATCCACAATCTCAA pLKO_005 1573 CDS 100% 4.950 3.465 N SRPK3 n/a
19 TRCN0000364064 GCTATTCCCGGGAGTTCTTCA pLKO_005 1536 CDS 100% 4.950 3.465 N SRPK3 n/a
20 TRCN0000196929 GCTCCTCAAATGGATCATCAA pLKO.1 556 CDS 100% 4.950 3.465 N SRPK3 n/a
21 TRCN0000007068 TGCAGATAAGATCAAGATCAA pLKO.1 1243 CDS 100% 4.950 3.465 N SRPK3 n/a
22 TRCN0000011066 CTCCAGCTCTCCGTGCCTTAA pLKO.1 1769 3UTR 100% 3.600 2.520 N SRPK3 n/a
23 TRCN0000011067 GCCACACAGTTCAGCGCCTTT pLKO.1 1649 CDS 100% 1.350 0.945 N SRPK3 n/a
24 TRCN0000368318 CGCCAGTTCTCCACAACCACA pLKO_005 1825 3UTR 100% 0.880 0.616 N SRPK3 n/a
25 TRCN0000199553 CGTGAGGGCTCTCGGAGAAAG pLKO.1 1904 3UTR 100% 0.000 0.000 N SRPK3 n/a
26 TRCN0000007066 CCACACCAAGTGCAAGATCAT pLKO.1 652 CDS 100% 4.950 2.970 N SRPK3 n/a
27 TRCN0000364065 GGATCATCAAGTCCAACTACC pLKO_005 567 CDS 100% 4.050 2.430 N SRPK3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014370.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488078 GCATTTTTACTGCACTATGGCCTT pLX_317 14.2% 99.8% 99.8% V5 (not translated due to prior stop codon) 774_776delGCA n/a
2 TRCN0000489375 GGTTACAGCATCAAGTGAACATCA pLX_317 20.5% 99.7% 99.6% V5 774_776delGCA;1701_1702insG n/a
3 ccsbBroadEn_08029 pDONR223 100% 99.7% 99.8% None 774_776delGCA;1014A>G n/a
4 ccsbBroad304_08029 pLX_304 0% 99.7% 99.8% V5 774_776delGCA;1014A>G n/a
5 TRCN0000480464 TATATAACCTGGTAATACAATGCC pLX_317 20.5% 99.7% 99.8% V5 774_776delGCA;1014A>G n/a
6 ccsbBroadEn_15037 pDONR223 97.1% 97.9% 41% None (many diffs) n/a
7 ccsbBroad304_15037 pLX_304 0% 97.9% 41% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000472667 AACACTAAATACATGGCAACCTCA pLX_317 20.4% 97.9% 41% V5 (not translated due to prior stop codon) (many diffs) n/a
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