Construct: ORF TRCN0000480470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008263.1_s317c1
- Derived from:
- ccsbBroadEn_08961
- DNA Barcode:
- CTACTGAGCTGATTTAACATGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSDMD (79792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79792 | GSDMD | gasdermin D | NM_001166237.1 | 99.9% | 100% | 492G>A |
| 2 | human | 79792 | GSDMD | gasdermin D | NM_024736.7 | 99.9% | 100% | 492G>A |
| 3 | human | 79792 | GSDMD | gasdermin D | XM_011517301.2 | 69.5% | 69.6% | 409_1041del;1125G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1518
- ORF length:
- 1452
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtcggccttt gagcgggtag tccggagagt ggtccaggag ctggaccatg 121 gtggggagtt catccctgtg accagcctgc agagctccac tggcttccag ccctactgcc 181 tggtggttag gaagccctca agctcatggt tctggaaacc ccgttataag tgtgtcaacc 241 tgtctatcaa ggacatcctg gagccggatg ccgcggaacc agacgtgcag cgtggcagga 301 gcttccactt ctacgatgcc atggatgggc agatacaggg cagcgtggag ctggcagccc 361 caggacaggc aaagatcgca ggcggggccg cggtgtctga cagctccagc acctcaatga 421 atgtgtactc gctgagtgtg gaccctaaca cctggcagac tctgctccat gagaggcacc 481 tgcggcagcc agaacacaaa gtcctgcagc agctgcgcag ccgcggggac aacgtgtacg 541 tggtgactga ggtgctacag acacagaagg aggtggaagt cacgcgcacc cacaagcggg 601 agggctcggg ccggttttcc ctgcccggag ccacgtgctt gcagggtgag ggccagggcc 661 atctgagcca gaagaagacg gtcaccatcc cctcaggcag caccctcgca ttccgggtgg 721 cccagctggt tattgactct gacttggacg tccttctctt cccggataag aagcagagga 781 ccttccagcc acccgcgaca ggccacaagc gttccacgag cgaaggcgcc tggccacagc 841 tgccctctgg cctctccatg atgaggtgcc tccacaactt cctgacagat ggggtccctg 901 cggagggggc gttcactgaa gacttccagg gcctacgggc agaggtggag accatctcca 961 aggaactgga gcttttggac agagagctgt gccagctgct gctggagggc ctggaggggg 1021 tgctgcggga ccagctggcc ctgcgagcct tggaggaggc gctggagcag ggccagagcc 1081 ttgggccggt ggagcccctg gacggtccag caggtgctgt cctggagtgc ctggtgttgt 1141 cctccggaat gctggtgccg gaactcgcta tccctgttgt ctacctgctg ggggcactga 1201 ccatgctgag tgaaacgcag cacaagctgc tggcggaggc gctGGAGTCG CAGACCCTGT 1261 TGGGGCCGCT CGAGCTGGTG GGCAGCCTCT TGGAGCAGAG TGCCCCGTGG CAGGAGCGCA 1321 GCACCATGTC CCTGCCCCCC GGGCTCCTGG GGAACAGCTG GGGCGAAGGA GCACCGGCCT 1381 GGGTCTTGCT GGACGAGTGT GGCCTAGAGC TGGGGGAGGA CACTCCCCAC GTGTGCTGGG 1441 AGCCGCAGGC CCAGGGCCGC ATGTGTGCAC TCTACGCCTC CCTGGCACTG CTATCAGGAC 1501 TGAGCCAGGA GCCCCACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTACTGA GCTGATTTAA 1681 CATGCGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt