Transcript: Human NM_001166237.1

Homo sapiens gasdermin D (GSDMD), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
GSDMD (79792)
Length:
2326
CDS:
704..2158

Additional Resources:

NCBI RefSeq record:
NM_001166237.1
NBCI Gene record:
GSDMD (79792)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001166237.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000180013 CCTTCTCTTCCCGGATAAGAA pLKO.1 1390 CDS 100% 5.625 7.875 N GSDMD n/a
2 TRCN0000180883 GAATGTGTACTCGCTGAGTGT pLKO.1 1057 CDS 100% 2.640 3.696 N GSDMD n/a
3 TRCN0000179394 GTGTGTCAACCTGTCTATCAA pLKO.1 868 CDS 100% 5.625 3.938 N GSDMD n/a
4 TRCN0000179101 CAGCACCTCAATGAATGTGTA pLKO.1 1045 CDS 100% 4.950 3.465 N GSDMD n/a
5 TRCN0000438254 GATGAGGTGCCTCCACAACTT pLKO_005 1498 CDS 100% 4.950 3.465 N GSDMD n/a
6 TRCN0000178784 CAACCTGTCTATCAAGGACAT pLKO.1 874 CDS 100% 4.050 2.835 N GSDMD n/a
7 TRCN0000437302 TACTGCCTGGTGGTTAGGAAG pLKO_005 812 CDS 100% 4.050 2.835 N GSDMD n/a
8 TRCN0000434253 TGGTTATTGACTCTGACTTGG pLKO_005 1365 CDS 100% 4.050 2.835 N GSDMD n/a
9 TRCN0000442600 ACTGAAGACTTCCAGGGCCTA pLKO_005 1553 CDS 100% 2.160 1.512 N GSDMD n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 530 5UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 531 5UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166237.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08961 pDONR223 100% 99.9% 100% None 492G>A n/a
2 ccsbBroad304_08961 pLX_304 0% 99.9% 100% V5 492G>A n/a
3 TRCN0000480470 CTACTGAGCTGATTTAACATGCGA pLX_317 19.3% 99.9% 100% V5 492G>A n/a
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