Construct: ORF TRCN0000480479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005173.1_s317c1
- Derived from:
- ccsbBroadEn_06527
- DNA Barcode:
- AGCTAAATCTCACTGTGTCTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LLGL2 (3993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | NM_001031803.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024626.1 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024627.1 | 99.7% | 99.6% | (many diffs) |
| 4 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_011524802.1 | 99.4% | 99.4% | (many diffs) |
| 5 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024625.1 | 99.3% | 99.2% | (many diffs) |
| 6 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | NM_004524.3 | 97.5% | 96.2% | (many diffs) |
| 7 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024630.1 | 97.5% | 96.2% | (many diffs) |
| 8 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024629.1 | 97.2% | 96% | (many diffs) |
| 9 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024628.1 | 97.1% | 95.9% | (many diffs) |
| 10 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_017024631.1 | 95.7% | 95.5% | (many diffs) |
| 11 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_001752508.1 | 83.1% | (many diffs) | |
| 12 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958003.1 | 79.8% | (many diffs) | |
| 13 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002957999.1 | 79.6% | (many diffs) | |
| 14 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958002.1 | 78.8% | (many diffs) | |
| 15 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958000.1 | 78.2% | (many diffs) | |
| 16 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958001.1 | 77.4% | (many diffs) | |
| 17 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958004.1 | 77.2% | (many diffs) | |
| 18 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XR_002958005.1 | 77.1% | (many diffs) | |
| 19 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | NM_001015002.1 | 34.6% | 34.1% | (many diffs) |
| 20 | human | 3993 | LLGL2 | LLGL scribble cell polarity... | XM_024450747.1 | 34.6% | 34.1% | (many diffs) |
| 21 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | NM_145438.2 | 86.3% | 90.4% | (many diffs) |
| 22 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XM_006533083.2 | 86.2% | 90.3% | (many diffs) |
| 23 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XM_006533084.2 | 86.1% | 90.1% | (many diffs) |
| 24 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XM_006533086.2 | 85.3% | 89.3% | (many diffs) |
| 25 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XM_006533085.2 | 85.2% | 89.4% | (many diffs) |
| 26 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XM_006533087.2 | 85.1% | 89.2% | (many diffs) |
| 27 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | NM_001252532.1 | 84.4% | 88.3% | (many diffs) |
| 28 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XR_388422.3 | 78.6% | (many diffs) | |
| 29 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XR_388421.2 | 72.8% | (many diffs) | |
| 30 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XR_388418.2 | 66.5% | (many diffs) | |
| 31 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XR_388420.2 | 66.2% | (many diffs) | |
| 32 | mouse | 217325 | Llgl2 | lethal giant larvae homolog... | XR_388419.2 | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3126
- ORF length:
- 3060
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcggttcctg aggccagggc atgaccctgt gcgggagagg ctcaagcggg 121 acctgttcca gtttaacaag acggtggagc atggcttccc gcaccagccc agcgccctcg 181 gctacagccc gtccctgcgc atcctggcca tcggcacccg ttctggagcc atcaagctct 241 acggagcccc aggcgtggag ttcatggggc tgcaccagga gaacaacgct gtgacgcaga 301 tccacctcct gcccggccag tgccagctgg tcaccctgct ggatgacaac agcctgcacc 361 tttggagcct gaaggtcaag ggcggggcat cggagctgca ggaggatgag agcttcacac 421 tgcgtggacc cccaggggct gcccccagtg ccacacagat caccgtggtc ctgccacatt 481 cctcctgcga gctgctctac ctgggcaccg agagtggcaa cgtgtttgtg gtgcagctgc 541 cagcttttcg tgcgctggag gaccggacca tcagctcgga cgcggtgctg cagcggttgc 601 cagaggaggc ccgccaccgg cgtgtgttcg agatggtgga ggcactgcag gagcaccctc 661 gagaccccaa ccagatcctg atcggctaca gccgaggcct cgttgtcatc tgggacctac 721 agggcagccg cgtgctctac cacttcctca gcagccagca actggagaac atctggtggc 781 agcgggacgg ccgcctgctc gtcagctgtc actctgacgg cagctactgc cagtggcccg 841 tgtccagcga agcccagcaa ccagagcccc tccgcagcct cgtgccttac ggtccctttc 901 cttgcaaagc gattaccaga atcctctggc tgaccactag gcaggggttg cccttcacca 961 tcttccaggg tggcatgcca cgggccagct acggggaccg ccactgcatc tcagtgatcc 1021 acgatggcca gcagacggcc ttcgacttca cctcccgtgt catcggcttc actgtcctca 1081 cagaggcaga ccctgcagcc acctttgacg acccctatgc cctggtggtg ctggctgagg 1141 aggagctggt ggtgattgac ctgcagacag caggctggcc accggtccag ctgccctacc 1201 tggcttctct gcactgttcc gccatcacct gctctcacca cgtctccaac atcccgctga 1261 agctgtggga gcggatcatt gccgccggca gccggcagaa cgcacacttc tccaccatgg 1321 agtggccaat tgatggtggc accagcctga ccccagcccc accccagagg gacctgctgc 1381 tcacagggca cgaggacggc acggtgcggt tctgggatgc ctcgggtgtc tgcctgcggc 1441 tgctctacaa actcagcact gtgcgcgtgt tcctcaccga cacggacccc aacgagaacc 1501 tcagtgccca gggcgaggac gagtggcccc cactccgcaa ggtgggctcc tttgacccct 1561 acagtgatga cccccggctg ggcatccaga agatcttcct ctgcaagtac agcggctacc 1621 tggctgtggc aggcacggca gggcaggtgc tggtactgga actgaatgac gaggcagcgg 1681 agcaggctgt ggagcaggtg gaggccgacc tgctgcagga ccaagagggc taccgctgga 1741 aggggcacga gcgcctggca gcccgctcag ggcccgtgcg ctttgagcct ggctttcagc 1801 ccttcgtgtt ggtgcagtgt cagcccccgg ctgtggtcac ctccttggcc ctgcactctg 1861 agtggcggct cgtggccttc ggcaccagcc atggctttgg cctctttgac caccagcagc 1921 ggcggcaggt ctttgttaag tgcacactgc accccagtga ccagctggcc ttggagggcc 1981 cactctcccg cgtcaagtcc ctcaagaagt ccttgcgtca gtcattccgc cggatgcgtc 2041 ggagccgggt gtccagccgg aagcggcacc cagctggccc cccaggagag gcacaggagg 2101 ggagtgccaa ggctgagcgg ccaggcctcc agaacatgga gctggcgcct gtgcagcgca 2161 agatcgaggc tcgctcggca gaggactcct tcacaggctt cgtccggacc ctgtactttg 2221 ctgacaccta cctgaaggac agctcccggc actgcccctc gctgtgggct ggcaccaatg 2281 ggggcaccat ctatgccttc tccctgcgtg tgcctcccgc cgagcggaga atggatgagt 2341 ctgtgcgggc agagcaggcc aaggagatcc agctgatgca ccgggcgccg gtggtgggca 2401 tcctggtgct cgacggacac agcgtacccc ttcctgagcc cctcgaagtg gcccatgatc 2461 tgtcgaagag ccctgacatg cagggaagcc accagctgct cgtcgtatca gaggagcagt 2521 tcaaggtgtt cacgctgccc aaggtgagtg ccaagctgaa gttgaagctg acggccctgg 2581 agggctcaag agtgcggcgg gtcagcgtgg cccacttcgg cagtcgtcga gccgaggact 2641 acggggagca ccacctggca gtccttacca acctgggcga catccaggtg gtctcgctgc 2701 ccctgctcaa gccccaggtg cgctacagct gcatccgccg ggaggacgtc agtggcatcg 2761 cctccTGCGT CTTCACCAAA TATGGCCAAG GCTTCTACCT GATCTCACCC TCGGAGTTTG 2821 AGCGCTTCTC TCTCTCCACC AAGTGGCTGG TGGAGCCCCG GTGTCTGGTG GATTCAGCAG 2881 AAACCAAGAA CCACCGCCCT GGTAACGGTG CGGGCCCCAA GAAGGCCCCG AGCCGAGCCA 2941 GGAACTCAGG GACTCAGAGT GATGGCGAGG AGAAGCAGCC CGGCCTGGTG ATGGAGCGCG 3001 CTCTGCTCAG TGATGAGAGA GTCCTGAAGG AAATCCAGAG CACACTGGAG GGAGACCGCG 3061 GGAGCGGCAA CTGGCGTTCA CATCGAGCGG CCGTGGGGTG CAGCCTCAGC AATGGCGGAG 3121 CAGAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3181 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT ATTTCGATTT 3241 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGCTAAATC TCACTGTGTC TCTTCACGCG 3301 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt