Construct: ORF TRCN0000480496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015309.1_s317c1
- Derived from:
- ccsbBroadEn_00806
- DNA Barcode:
- GAATTAACCATGATCGGTTTGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR5A (3361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3361 | HTR5A | 5-hydroxytryptamine recepto... | NM_024012.3 | 100% | 100% | |
| 2 | mouse | 15563 | Htr5a | 5-hydroxytryptamine (seroto... | NM_008314.2 | 84.4% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatttacct gtgaacctaa cctccttttc cctctccacc ccctcccctt 121 tggagaccaa ccacagcctc ggcaaagacg acctgcgccc cagctcgccc ctgctctcgg 181 tcttcggagt gcttattctc accttgctgg gctttctggt ggcggcgacg ttcgcctgga 241 acctgctggt gctggcgacc atcctccgtg tacgcacctt ccaccgcgtg ccccacaacc 301 tggtggcatc catggccgtc tcggatgtcc tggtggccgc gctggtcatg ccgctgagcc 361 tggtgcacga gctgtccggg cgccgctggc agctaggtcg gaggctgtgc cagctttgga 421 tcgcgtgcga cgtgctttgc tgcacggcca gcatctggaa cgtgacggcc atagccctgg 481 accgctactg gtccatcacg cgccacatgg aatacacgct ccgcacccgc aagtgcgtct 541 ccaacgtcat gatcgcgctc acctgggcac tctccgctgt catctctctg gccccgctgc 601 tttttggctg gggagagacg tactctgagg gcagcgagga gtgccaggta agccgcgagc 661 cttcctacgc cgtgttctcc accgtaggcg ccttctacct gccgctctgt gtggtgctct 721 tcgtgtactg gaagatctac aaggctgcca agttccgcgt gggctccagg aagaccaata 781 gcgtctcacc catatccGAA GCTGTGGAGG TGAAGGACTC TGCCAAACAG CCCCAGATGG 841 TGTTCACGGT CCGCCACGCC ACCGTCACCT TCCAGCCAGA AGGGGACACG TGGCGGGAGC 901 AGAAGGAGCA GCGGGCCGCC CTCATGGTGG GCATCCTCAT TGGCGTGTTC GTGCTCTGCT 961 GGATCCCCTT CTTTCTCACC GAGCTCATCA GTCCCCTCTG CTCCTGTGAC ATCCCCGCCA 1021 TCTGGAAAAG CATCTTCCTG TGGCTTGGCT ACTCCAACTC CTTCTTTAAC CCCCTGATCT 1081 ATACGGCTTT CAACAAGAAC TACAACAGCG CCTTCAAGAA CTTCTTTTCT AGGCAACACT 1141 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAGAATT AACCATGATC GGTTTGGTTA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt