Construct: ORF TRCN0000480503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007483.1_s317c1
- Derived from:
- ccsbBroadEn_05420
- DNA Barcode:
- CTTCGCGTCTTCCTGCTTAGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4E3 (317649)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 317649 | EIF4E3 | eukaryotic translation init... | NM_001134649.2 | 100% | 100% | |
| 2 | human | 317649 | EIF4E3 | eukaryotic translation init... | NM_001134650.1 | 100% | 100% | |
| 3 | human | 317649 | EIF4E3 | eukaryotic translation init... | NM_001282886.2 | 100% | 100% | |
| 4 | human | 317649 | EIF4E3 | eukaryotic translation init... | NM_173359.5 | 100% | 100% | |
| 5 | human | 317649 | EIF4E3 | eukaryotic translation init... | XM_017006282.2 | 100% | 100% | |
| 6 | human | 317649 | EIF4E3 | eukaryotic translation init... | XM_011533651.3 | 62.9% | 53.3% | (many diffs) |
| 7 | human | 317649 | EIF4E3 | eukaryotic translation init... | NM_001134651.2 | 52.6% | 52.6% | 1_318del |
| 8 | mouse | 66892 | Eif4e3 | eukaryotic translation init... | NM_025829.4 | 50.7% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GAGAGGAGAG AGGCGACCAC TTTGGGAAGA GGAGAGTAAT GCAAAGGGTG 121 GCGTATGGAA GATGAAAGTC CCCAAGGACA GCACGTCCAC AGTTTGGAAA GAGTTGCTGT 181 TAGCAACCAT CGGGGAACAG TTCACAGACT GTGCCGCAGC AGATGATGAA GTAATAGGAG 241 TTAGTGTCAG TGTTCGGGAC CGAGAAGACG TCGTCCAAGT CTGGAATGTA AATGCCTCTT 301 TAGTGGGTGA AGCGACTGTT TTAGAAAAGA TCTATGAACT TCTGCCCCAC ATAACTTTTA 361 AAGCAGTATT TTATAAACCC CATGAAGAGC ATCATGCTTT TGAAGGTGGA CGTGGAAAAC 421 ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGACT TCGCGTCTTC CTGCTTAGGG TGACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt