Construct: ORF TRCN0000480510
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002651.1_s317c1
- Derived from:
- ccsbBroadEn_01650
- DNA Barcode:
- AGAATGCTCGGCGCCACTGGGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCEA1 (6917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480510
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6917 | TCEA1 | transcription elongation fa... | NM_006756.4 | 100% | 100% | |
| 2 | human | 6917 | TCEA1 | transcription elongation fa... | NM_201437.3 | 93% | 93% | 62_63ins63 |
| 3 | human | 6917 | TCEA1 | transcription elongation fa... | XM_011517578.1 | 88.3% | 88.3% | 0_1ins105 |
| 4 | human | 6917 | TCEA1 | transcription elongation fa... | XM_006716467.1 | 84.3% | 84.3% | 0_1ins141 |
| 5 | human | 6917 | TCEA1 | transcription elongation fa... | NR_109901.2 | 28.6% | (many diffs) | |
| 6 | mouse | 21399 | Tcea1 | transcription elongation fa... | NM_011541.4 | 92.2% | 96.6% | (many diffs) |
| 7 | mouse | 21399 | Tcea1 | transcription elongation fa... | NM_001159750.1 | 92% | 96.6% | (many diffs) |
| 8 | mouse | 21399 | Tcea1 | transcription elongation fa... | NM_001159751.1 | 86.5% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 972
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggacgaa gtggtccgct ttgccaagaa gatggacaag atggtgcaga 121 agaagaacgc ggctggagca ttggatttgc taaaggagct taagaatatt cctatgaccc 181 tggaattact gcagtccaca agaatcggaa tgtcagttaa tgctattcgc aagcagagta 241 cagatgagga agttacatct ttggcaaagt ctctcatcaa atcctggaaa aaattattag 301 atgggccatc aactgagaaa gaccttgacg aaaagaagaa agaacctgca attacatcgc 361 agaacagccc tgaggcaaga gaagaaagta cttccagcgg caatgtaagc aacagaaagg 421 atgagacaaa tgctcgagat acttatgttt catcctttcc tcgggcacca agcacttctg 481 attctgtgcg gttgaagtgt agggagatgc ttgctgcagc tcttcgaaca ggggatgact 541 aCATTGCAAT TGGAGCTGAT GAGGAAGAAT TAGGATCTCA AATTGAAGAA GCTATATATC 601 AAGAAATAAG GAATACAGAC ATGAAATACA AAAATAGAGT ACGAAGTAGG ATATCAAATC 661 TTAAAGATGC AAAAAATCCA AATTTAAGGA AAAATGTCCT CTGTGGGAAT ATTCCTCCTG 721 ACTTATTTGC TAGAATGACA GCAGAGGAAA TGGCTAGTGA TGAGCTGAAA GAGATGCGGA 781 AAAACTTGAC CAAAGAAGCC ATCAGAGAGC ATCAGATGGC CAAGACTGGT GGGACCCAGA 841 CTGACTTGTT CACATGTGGC AAATGTAAAA AGAAGAATTG CACTTACACA CAGGTACAAA 901 CCCGTAGTGC TGATGAACCA ATGACAACAT TTGTTGTCTG TAATGAATGT GGAAATCGAT 961 GGAAGTTCTG TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGA ATGCTCGGCG CCACTGGGGG 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t