Construct: ORF TRCN0000480512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001469.1_s317c1
- Derived from:
- ccsbBroadEn_03217
- DNA Barcode:
- AGAATCAGAGAGGTTAAACAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SBDS (51119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51119 | SBDS | SBDS ribosome maturation fa... | NM_016038.4 | 100% | 100% | |
| 2 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_001588.2 | 62.1% | (many diffs) | |
| 3 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024109.1 | 50.8% | (many diffs) | |
| 4 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024110.1 | 41.1% | (many diffs) | |
| 5 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024111.1 | 34.4% | (many diffs) | |
| 6 | mouse | 66711 | Sbds | SBDS ribosome assembly guan... | NM_023248.2 | 88.2% | 97.2% | (many diffs) |
| 7 | mouse | 66711 | Sbds | SBDS ribosome assembly guan... | XM_006504490.2 | 48.8% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 816
- ORF length:
- 750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gatcttcacc cccaccaacc agatccgcct aaccaatgtg gccgtggtac 121 ggatgaagcg tgccgggaag cgcttcgaaa tcgcctgcta caaaaacaag gtcgtcggct 181 ggcggagcgg cgtggaaaaa gacctcgatg aagttctgca gacccactca gtgtttgtaa 241 atgtttctaa aggtcaggtt gccaaaaagg aagatctcat cagtgcgttt ggaacagatg 301 accaaactga aatctgtaag cagattttga ctaaaggaga agttcaagta tcagataaag 361 aaagacacac acaactggag cagatgttta gggacattgc aactattgtg gcagacaaat 421 gtgtgaatcc tgaaacaaag agaccataca ccgtgaTCCT TATTGAGAGA GCCATGAAGG 481 ACATCCACTA TTCGGTGAAA ACCAACAAGA GTACAAAACA GCAGGCTTTG GAAGTGATAA 541 AGCAGTTAAA AGAGAAAATG AAGATAGAAC GTGCTCACAT GAGGCTTCGG TTCATCCTTC 601 CAGTCAATGA AGGCAAGAAG CTGAAAGAAA AGCTCAAGCC ACTGATCAAG GTCATAGAAA 661 GTGAAGATTA TGGCCAACAG TTAGAAATCG TATGTCTGAT TGACCCGGGC TGCTTCCGAG 721 AAATTGATGA GCTAATAAAA AAGGAAACTA AAGGCAAAGG TTCTTTGGAA GTACTCAATC 781 TGAAAGATGT AGAAGAAGGA GATGAGAAAT TTGAATGCCC AACTTTCTTG TACAAAGTGG 841 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 901 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 961 AAGAATCAGA GAGGTTAAAC AAAACACGCG TTAAGTCgac aatcaacctc tggattacaa 1021 aatttgtgaa agatt