Construct: ORF TRCN0000480521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015826.1_s317c1
- Derived from:
- ccsbBroadEn_07658
- DNA Barcode:
- AAGGACGGTTGATCGAGCTACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAS2L1 (10634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | NM_001362985.2 | 99.9% | 99.8% | 30C>T;1205G>N |
| 2 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | NM_006478.5 | 99.9% | 99.8% | 30C>T;1205G>N |
| 3 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | NM_152236.3 | 99.9% | 99.8% | 30C>T;1205G>N |
| 4 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | XM_011529825.1 | 99.9% | 99.8% | 30C>T;1205G>N |
| 5 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | XM_017028533.1 | 99.9% | 99.8% | 30C>T;1205G>N |
| 6 | human | 10634 | GAS2L1 | growth arrest specific 2 li... | NM_001278730.2 | 66.6% | 66.6% | 30C>T;1006_1007ins681 |
| 7 | mouse | 78926 | Gas2l1 | growth arrest-specific 2 li... | NM_001190408.1 | 82.5% | 86.2% | (many diffs) |
| 8 | mouse | 78926 | Gas2l1 | growth arrest-specific 2 li... | NM_144560.3 | 82.5% | 86.2% | (many diffs) |
| 9 | mouse | 78926 | Gas2l1 | growth arrest-specific 2 li... | NM_030228.3 | 41.7% | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2109
- ORF length:
- 2043
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agacccagtg gcgggcatcg cgggttcggc ggccaagagc gtgcggccat 121 ttcgctccag tgaggcctac gtggaggcca tgaaggagga cctggccgag tggctcaatg 181 ccttgtacgg cctgggtctc ccgggtggtg gcgatggctt cctgacaggg ctggccacgg 241 gcacgaccct gtgccaacat gccaacgccg tgaccgaggc tgcccgtgca ttggcagccg 301 cccgcccggc ccgaggtgtg gccttccagg cgcacagtgt agtgcctggc tccttcatgg 361 cgcgcgacaa cgtggccacc ttcatcggct ggtgccgcgt ggagctgggt gtgccggagg 421 tgctcatgtt tgagactgag gacctggtgc tgcgcaagaa cgagaagagc gtggtgctgt 481 gcctgctgga ggtggcgcgg cgtggggcac gcctgggcct gctggcccca cgcctcgtgc 541 agtttgagca ggagattgag cgggagctgc gtgctgcacc cccagccccc aacgcccctg 601 ccgctgggga ggacaccact gaaaccgccc ccgcaccagg gactcctgcc cgcggccccc 661 gcatgacacc cagcgacctg cgcaacctcg acgagctggt gagggagatt ctgggccgct 721 gcacctgccc tgaccagttt cccatgatca aggtctcaga ggggaagtac cgtgtggggg 781 actcgagcct gctcatcttt gtgcgggtgc tgaggagcca cgtgatggtg cgagtgggtg 841 gtggctggga cacgctggag cattacctgg acaagcacga cccgtgccgc tgctcctcca 901 ctgctcatcg cccaccccag ccgagggtct gcaccttttc tccacagagg gtgtcgccca 961 ccaccagtcc ccgccctgct agcccagtcc ctgggagtga gcgccggggc tcccggcctg 1021 agatgactcc cgttagctta cgaagcacaa aggaggggcc cgagacccca cccaggcccc 1081 gggatcagct gcccccccat ccccgctccc gccgctactc cggggacagt gactcctcag 1141 cctcctccgc ccagagcggc ccccttggta cccgcagtga tgacacaggc actggccccc 1201 ggagggagcg acccagccgg cggctgacca caggcacccc ggcctctccg agacggcctc 1261 ctgccctgcn cagccagtcc cgagaccggc tggatcgcgg ccggccccgg ggggccccag 1321 gaggcagggg agcccagctg tcggtcccca gccctgcccg gcgggcccgg agccagagcc 1381 gcgaggagca ggctgtgctg cttgtgcgca gggatcgaga cgggcagcac tcatgggtgc 1441 caaggggcag gggcagtggg ggctcgggca ggagcacccc ccagactccc cgtgcccgca 1501 gccctgcagc accccggctt tcccgggtct ccagccccag tccagagttg ggcaccacac 1561 cggccagcat cttccgcaca cccctgcagc tcgacccgca gcaggagcag cagctgttcc 1621 ggcgcctgga agaggagttc ctggccaatg cccgggccct tgaggctgtt gctagcgtga 1681 cccccactgg accagcccct gacccagcTC GGGCCCCCGA CCCTCCAGCT CCTGACTCTG 1741 CCTATTGTTC CTCCAGTTCC TCCTCTTCGT CCCTCAGCGT CCTGGGTGGC AAATGTGGCC 1801 AACCTGGGGA CTCTGGCCGG ACGGCCAATG GGCTGCCTGG GCCCCGAAGC CAAGCCCTTT 1861 CCAGCTCCTC CGATGAAGGC AGCCCCTGCC CTGGCATGGG GGGGCCACTA GATGCACCTG 1921 GGAGCCCCCT GGCTTGCACT GAACCCTCGA GGACCTGGGC ACGGGGTCGG ATGGACACAC 1981 AGCCAGACCG TAAACCCTCA CGTATCCCCA CGCCTCGGGG CCCCCGCCGC CCCTCCGGAC 2041 CCGCAGAGCT GGGGACATGG CATGCCCTGC ACTCAGTCAC CCCGAGGGCT GAGCCAGATT 2101 CCTGGATGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2161 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2221 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGGAC GGTTGATCGA GCTACATCAC 2281 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt