Construct: ORF TRCN0000480531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012799.1_s317c1
- Derived from:
- ccsbBroadEn_15873
- DNA Barcode:
- GACTCCGATGACCGGCAACATGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TENT5C (54855)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480531
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54855 | TENT5C | terminal nucleotidyltransfe... | NM_017709.4 | 100% | 100% | |
| 2 | mouse | 74645 | Fam46c | family with sequence simila... | NM_001142952.1 | 89.9% | 95.9% | (many diffs) |
| 3 | mouse | 74645 | Fam46c | family with sequence simila... | XM_006502212.3 | 89.9% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1242
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaggag agcagctgta ccagggattg catgtccttc agcgtgctca 121 actgggatca ggttagccgg ctgcatgagg tcctcactga agttgtacct atccacggac 181 gaggcaactt tccaaccttg gagataactc tgaaggacat cgtccagacc gtccgcagtc 241 ggctggagga ggcaggcatc aaagtgcacg acgtccggct gaatggctcc gcagctggcc 301 acgttttggt caaagacaat ggcttgggct gcaaagacct ggacctaatc ttccatgtgg 361 ctcttccaac agaggcagaa tttcagctgg ttagagatgt ggttctgtgt tcccttctga 421 acttcctgcc agagggtgtg aacaagctca aaatcagtcc agtcactctg aaggaggcat 481 atgtgcagaa gctagtgaag gtttgcacgg acactgaccg ctggagcctg atctccctct 541 ccaacaagaa cgggaagaac gtggagctga agtttgtcga ctccattcgg cgtcagtttg 601 agttcagtgt ggactctttc caaatcatcc tggattcttt gcttttcttc tatgactgtt 661 ccaataatcc catctctgag cacttccacc ccaccgtgat tggggagagc atgtacgggg 721 actttgagga agcttttgac catctgcaga acagactgat cgccaccaag aacccagaag 781 aaatcagagg cgggggactt ctcaagtaca gcaaccttct tgtgcgggac ttcaggccca 841 cagaccagga agaaatcaaa actctagagc gctacatgtg ctccaggttc TTCATCGACT 901 TCCCGGACAT CCTTGAACAG CAGAGGAAGT TGGAGACTTA CCTTCAAAAC CACTTCGCTG 961 AAGAAGAGAG AAGCAAGTAC GACTACCTCA TGATCCTTCG CAGGGTGGTG AACGAGAGCA 1021 CCGTGTGTCT CATGGGGCAT GAACGCAGGC AGACTCTGAA CCTCATCTCC CTCCTGGCCT 1081 TGCGTGTGCT GGCGGAACAA AACATCATCC CCAGTGCCAC CAACGTCACC TGTTACTACC 1141 AGCCGGCCCC TTACGTCAGT GATGGCAACT TCAGCAACTA CTACGTTGCC CATCCTCCAG 1201 TCACCTACAG CCAGCCTTAC CCTACCTGGC TGCCCTGTAA CTTGCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGAC TCCGATGACC GGCAACATGA AACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t