Construct: ORF TRCN0000480537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008991.1_s317c1
- Derived from:
- ccsbBroadEn_01212
- DNA Barcode:
- CCTTGCAACCTTGGTCTTCATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKP2 (5318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5318 | PKP2 | plakophilin 2 | NM_001005242.2 | 100% | 100% | |
| 2 | human | 5318 | PKP2 | plakophilin 2 | NM_004572.3 | 95% | 95% | 1378_1509del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2580
- ORF length:
- 2511
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagccccc ggcgccccag ctgagtacgg ctacatccgg accgtcctgg 121 gccagcagat cctgggacaa ctggacagct ccagcctggc gctgccctcc gaggccaagc 181 tgaagctggc ggggagcagc ggccgcggcg gccagacagt caagagcctg cggatccagg 241 agcaggtgca gcagaccctc gcccggaagg gccgcagctc cgtgggcaac ggaaatcttc 301 accgaaccag cagtgttcct gagtatgtct acaacctaca cttggttgaa aatgattttg 361 ttggaggccg ttcccctgtt cctaaaacct atgacatgct aaaggctggc acaactgcca 421 cttatgaagg tcgctgggga agaggaacag cacagtacag ctcccagaag tccgtggaag 481 aaaggtcctt gaggcatcct ctgaggagac tggagatttc tcctgacagc agcccggaga 541 gggctcacta cacgcacagc gattaccagt acagccagag aagccaggct gggcacaccc 601 tgcaccacca agaaagcagg cgggccgccc tcctagtgcc accgagatat gctcgttccg 661 agatcgtggg ggtcagccgt gctggcacca caagcaggca gcgccacttt gacacatacc 721 acagacagta ccagcatggc tctgttagcg acaccgtttt tgacagcatc cctgccaacc 781 cggccctgct cacgtacccc aggccaggga ccagccgcag catgggcaac ctcttggaga 841 aggagaacta cctgacggca gggctcactg tcgggcaggt caggccgctg gtgcccctgc 901 agcccgtcac tcagaacagg gcttccaggt cctcctggca tcagagctcc ttccacagca 961 cccgcacgct gagggaagct gggcccagtg tcgccgtgga ttccagcggg aggagagcgc 1021 acttgactgt cggccaggcg gccgcagggg gaagtgggaa tctgctcact gagagaagca 1081 ctttcactga ctcccagctg gggaatgcag acatggagat gactctggag cgagcagtga 1141 gtatgctcga ggcagaccac atgctgccat ccaggatttc tgctgcagct actttcatac 1201 agcacgagtg cttccagaaa tctgaagctc ggaagagggt taaccagctt cgtggcatcc 1261 tcaagcttct gcagctccta aaagttcaga atgaagacgt tcagcgagct gtgtgtgggg 1321 ccttgagaaa cttagtattt gaagacaatg acaacaaatt ggaggtggct gaactaaatg 1381 gggtacctcg gctgctccag gtgctgaagc aaaccagaga cttggagact aaaaaacaaa 1441 taacaggttt gctgtggaat ttgtcatcta atgacaaact caagaatctc atgataacag 1501 aagcattgct tacgctgacg gagaatatca tcatcccctt ttctgggtgg cctgaaggag 1561 actacccaaa agcaaatggt ttgctcgatt ttgacatatt ctacaacgtc actggatgcc 1621 taagaaacat gagttctgct ggcgctgatg ggagaaaagc gatgagaaga tgtgacggac 1681 tcattgactc actggtccat tatgtcagag gaaccattgc agattaccag ccagatgaca 1741 aggccacgga gaattgtgtg tgcattcttc ataacctctc ctaccagctg gaggcagagc 1801 tcccagagaa atattcccag aatatctata ttcaaaaccg gaatatccag actgacaaca 1861 acaaaagtat tggatgtttt ggcagtcgaa gcaggaaagt aaaagagcaa taccaggacg 1921 tgccgatgcc ggaggaaaag agcaacccca agggcgtgga gtggctgtgg cattccattg 1981 ttataaggat gtatctgtcc ttgatcgcca aaagtgtccg caactacaca caagaagcat 2041 ccttaggagc tctgcagaac ctcacggccg gaagtggacc aatgccgaca tcagtggctc 2101 agacagttgt ccagaaggaa agtggcctgc agcacacccg aaagatgctg catgttggtg 2161 acccaagtgt GAAAAAGACA GCCATCTCGC TGCTGAGGAA TCTGTCCCGG AATCTTTCTC 2221 TGCAGAATGA AATTGCCAAA GAAACTCTCC CTGATTTGGT TTCCATCATT CCTGACACAG 2281 TCCCGAGTAC TGACCTTCTC ATTGAAACTA CAGCCTCTGC CTGTTACACA TTGAACAACA 2341 TAATCCAAAA CAGTTACCAG AATGCACGCG ACCTTCTAAA CACCGGGGGC ATCCAGAAAA 2401 TTATGGCCAT TAGTGCAGGC GATGCCTATG CCTCCAACAA AGCAAGTAAA GCTGCTTCCG 2461 TCCTTCTGTA TTCTCTGTGG GCACACACGG AACTGCATCA TGCCTACAAG AAGGCTCAGT 2521 TTAAGAAGAC AGATTTTGTC AACAGCCGGA CTGCCAAAGC CTACCACTCC CTTAAAGACT 2581 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2641 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2701 TTTATATATC TTGTGGAAAG GACGACCTTG CAACCTTGGT CTTCATCCCA CGCGTTAAGT 2761 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt