Construct: ORF TRCN0000480546

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000915.1_s317c1
Derived from:
ccsbBroadEn_06603
DNA Barcode:
CATAATTTAAATACTTACTCAGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MUC1 (4582)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480546
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4582 MUC1 mucin 1, cell surface assoc... NM_001018017.3 99.8% 100% 66G>A
2 human 4582 MUC1 mucin 1, cell surface assoc... NM_001018016.3 96.4% 96.5% 55_81del;93G>A
3 human 4582 MUC1 mucin 1, cell surface assoc... NM_002456.6 93.2% 93.4% 66G>A;160_213del
4 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204287.2 90.3% 90.4% 55_81del;93G>A;187_240del
5 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204294.2 90% 85.4% 66G>A;159_160insTTTAATTCCTCTCTGGAAG;208_209ins56
6 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204291.1 87.7% 87.8% 55_81del;93G>A;186_187ins69
7 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204292.1 86.9% 82.5% (many diffs)
8 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204289.2 86.6% 86.7% 55_81del;93G>A;186_187ins78
9 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204290.2 85% 85% 55_56ins36;123_124ins78
10 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044390.3 79.4% 68.2% 66G>A;159_160insTTTAATTCCTCTCTGGAAG;264_265ins137
11 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204288.2 79.4% 56.6% (many diffs)
12 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204296.2 76.7% 65.9% (many diffs)
13 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204295.1 68% 68.1% 55_81del;93G>A;254_255ins225
14 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204293.2 65.8% 50.5% (many diffs)
15 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044393.3 61.8% 26.7% (many diffs)
16 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044391.3 58.6% 58.8% 66G>A;226_227ins315
17 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044392.3 56.6% 56.8% 55_81del;93G>A;253_254ins315
18 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204285.2 53.6% 53.6% 66G>A;160_819del
19 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204297.2 53% 53.1% (many diffs)
20 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204286.1 52.6% 52.6% 55_81del;93G>A;187_846del
21 human 4582 MUC1 mucin 1, cell surface assoc... NM_001371720.1 19.4% 17.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
834
ORF length:
765
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacaccgggc acccagtctc ctttcttcct gctgctgctc ctcacagtgc
121ttacagttgt tacaggttct ggtcatgcaa gctctacccc aggtggagaa aaggagactt
181cggctaccca gagaagttca gtgcccagct ctactgagaa gaatgctttt aattcctctc
241tggaagatcc cagcaccgac tactaccaag agctgcagag agacatttct gaaatgtttt
301tgcagattta taaacaaggg ggttttctgg gcctctccaa tattaagttc aggccaggat
361ctgtggtggt acaattgact ctggccttcc gagaaggtac catcaatgtc cacgacgtgg
421agacacagtt caatcagtat aaaacggaag cagcctctcg atataacctg acgatcTCAG
481ACGTCAGCGT GAGTGATGTG CCATTTCCTT TCTCTGCCCA GTCTGGGGCT GGGGTGCCAG
541GCTGGGGCAT CGCGCTGCTG GTGCTGGTCT GTGTTCTGGT TGCGCTGGCC ATTGTCTATC
601TCATTGCCTT GGCTGTCTGT CAGTGCCGCC GAAAGAACTA CGGGCAGCTG GACATCTTTC
661CAGCCCGGGA TACCTACCAT CCTATGAGCG AGTACCCCAC CTACCACACC CATGGGCGCT
721ATGTGCCCCC TAGCAGTACC GATCGTAGCC CCTATGAGAA GGTTTCTGCA GGTAATGGTG
781GCAGCAGCCT CTCTTACACA AACCCAGCAG TGGCAGCCAC TTCTGCCAAC TTGTTGCCAA
841CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
901ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
961TATCTTGTGG AAAGGACGAC ATAATTTAAA TACTTACTCA GTTACGCGTT AAGTCgacaa
1021tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)