Construct: ORF TRCN0000480559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010007.1_s317c1
- Derived from:
- ccsbBroadEn_03714
- DNA Barcode:
- CGGATCATAGTTGCTGAAGCTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERTAD4 (56256)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480559
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56256 | SERTAD4 | SERTA domain containing 4 | NM_019605.5 | 100% | 100% | |
| 2 | human | 56256 | SERTAD4 | SERTA domain containing 4 | XM_024448435.1 | 100% | 100% | |
| 3 | human | 56256 | SERTAD4 | SERTA domain containing 4 | NM_001354173.2 | 36.6% | 29.6% | (many diffs) |
| 4 | human | 56256 | SERTAD4 | SERTA domain containing 4 | XR_921894.2 | 20.9% | 1_145del;1214_5098del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tctggttctg tccatgaata gattctgcga gcccattgtc tcggaaggag 121 ctgctgaaat tgctgggtac caaacactat gggaggctga cagctacgga ggcccaagcc 181 ccccagggcc agcacaagct cctttgcagg gagaccgggg agctggtccc ccactggcag 241 gatcacatta caggggaatt tcaaatccta taacaacatc caagatcaca tactttaaga 301 ggaagtatgt ggaagaagag gattttcacc caccactcag cagctgtagc cataaaacca 361 tctcaatttt tgaggaacga gcccacatcc tttatatgtc cttagaaaag ctaaagttta 421 tcgatgatcc tgaagtgtac ctccgaagat ctgtccttat aaacaatttg atgaaaagga 481 tccatggaga aattatcatg cagaataact ggtgcttccc tgcctgctct ttcaatggca 541 cctctgccca agagtggttt atggctcaag actgccctta ccgaaaacga ccacggatgg 601 ccaaagagga atgtgaaaag tttcatgcct gctgctttta ccaagaatgt ggtggccact 661 acctaaattt acccctttct gtcaatgcta atgttggaag tgcctccact gctgcctcct 721 ctccctccgc ctcttcttcc tcctcaTCTT CCTCTTCCTC TCCCCCTTTG CCTTTACCGA 781 GTTGTTCCCG CCAGGTGGAT TTTGATGTAG GTAGTGCATC TATTTACAAG AGTGATGGCC 841 AGATACCTGC CAATGAAATC TTTGTCACTA ATGTCAGATC ACTTGGTGTT CAGGAAAAGG 901 CCAAATTAAA TGATGAGAAA GCAAATGATG ACACCAACAG AGATGGTGGC CCCCTCAGCC 961 ACGAACCTGT GGGAAATGAC CTTGCTTTTG AGTGCAAAGG CCAATTTTAT GATTATTTTG 1021 AGACCGGATA TAATGAAAGA AACAATGTAA ATGAATCTTG GAAAAAGTCC TTACGGAAAA 1081 AGGAGGCTTC ACCACCAAGT AACAAACTGT GCTGCAGCAA AGGAAGTAAA ATATGCCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAC GGATCATAGT TGCTGAAGCT AAGACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att