Construct: ORF TRCN0000480563
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007435.1_s317c1
- Derived from:
- ccsbBroadEn_10785
- DNA Barcode:
- AAGCCTACTAACCAGATTTTATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAH6 (1768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480563
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_017003524.1 | 18.3% | 18.3% | (many diffs) |
| 2 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532660.1 | 17.8% | 17.8% | (many diffs) |
| 3 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532657.1 | 10.8% | 10.8% | (many diffs) |
| 4 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_017003522.2 | 10.8% | 10.8% | (many diffs) |
| 5 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_017003523.2 | 10.8% | 10.8% | (many diffs) |
| 6 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532655.2 | 10.6% | 10.6% | (many diffs) |
| 7 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532654.1 | 10% | 10% | (many diffs) |
| 8 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_017003521.1 | 9.9% | 9.9% | (many diffs) |
| 9 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532653.1 | 9.8% | 9.8% | (many diffs) |
| 10 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532652.1 | 9.8% | 9.8% | (many diffs) |
| 11 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | NM_001370.2 | 9.7% | 9.7% | (many diffs) |
| 12 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_006711956.2 | 9.7% | 9.7% | (many diffs) |
| 13 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532649.3 | 9.7% | 9.7% | (many diffs) |
| 14 | human | 1768 | DNAH6 | dynein axonemal heavy chain 6 | XM_011532650.3 | 9.7% | 9.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1293
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacatataca gaacaggcca gcaaaaggca ttattgcatg aggctgacgt 121 gctttattcg tctaaacgac tatctaattg agaacacaat gcacatctta acggtaaatg 181 ctgttaattc gcttttgaac catctcactg acaagctaaa acgaacacct tcagcagatg 241 tcattcagaa atggattact gaagagaagc ctgaagtccc tgataaaaag gggaccctta 301 tggtggaaaa gcaagaagaa gatgaatctc tcatccccat gtttctcaca gaactaatgt 361 tgacagtcca gtcactgctc tttgagcctt ctctggaaga ctttctggat ggtattttgg 421 gtgcagttaa tcactgtcaa aacactgtgt tatcagttcc taatctcgtg cctgattcgt 481 attttgatgc tttcaccagc ccttatatta acaacaaact tgaaggaaaa acctgtggaa 541 ctgggccaag tttagcagca gtatttgagg atgataagaa ttttcacaca attatttctc 601 aaataaagga aaccattcag gcagcatttg aatcagcccg catctatgca gctacctttg 661 aaaagttcca gatattcttc aaggaaaatg aaagtcttga tttacaagct cttaaacttc 721 aggaacctga tattaacttt tttagtgaac aactggaaaa atatcacaaa cagcacaagg 781 acgcagtagc gctcagaccc accagaaatg taggattgct gctcattgat actaggcttc 841 taagagaaaa attaattcca tcacctttgc gatgcttaga ggtgctaaat tttatgcttc 901 ctcGTCAAAG CAAGAAAAAA GTGGATGCCA TTATCTTTGA GGCACAAGAT GCAGAGTATA 961 AACTTGAGTT TGTTCCAACT ACTACCACAG AATATGTTCA TAGCTTATTA TTTCTTGATG 1021 AAATTCAGGA ACGGATTGAA AGCCTTGAAG ATGAGGGGAA TATAGTGACT CAAATGTACA 1081 AGCTTATGGA ACAATATCAG GTGCCCACAC CTCCTGAAGA CTTTGCTGTT TTTGCAACTA 1141 TGAAGCCATC CATTGTTGCT GTTCGGAATG CCATTGATAA ATCAGTGGGT GATAGAGAAT 1201 CAAGCATTAA GCAATTTTGT GTGCATTTGG GTAGTGATCT TGAAGAATTA AACAACGAAG 1261 TGAATGAAGT AAAACTGCAA GCACAGGTAA GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1441 GCCTACTAAC CAGATTTTAT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt