Construct: ORF TRCN0000480572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003847.3_s317c1
- Derived from:
- ccsbBroadEn_14716
- DNA Barcode:
- GTATTGTACTGAACGTTCCGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME3 (4832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | NM_002513.2 | 99.8% | 100% | 282A>T |
| 2 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_005255332.4 | 97.4% | 75% | 282A>T;384_385insGTTGGCAA;495_496insTGAG |
| 3 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_011522503.2 | 96.1% | 58.8% | 177_187del;293A>T;395_396insGTTGGCAA |
| 4 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_011522504.2 | 76.4% | 33.3% | 177_187del;293A>T;408_409ins110 |
| 5 | mouse | 79059 | Nme3 | NME/NM23 nucleoside diphosp... | NM_019730.2 | 82.8% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ctgcctggtg ctgaccatct tcgctaacct cttccccgcg gccTGCACCG 121 GCGCACACGA ACGCACCTTC CTGGCCGTGA AGCCGGACGG CGTGCAGCGG CGGCTGGTGG 181 GCGAGATTGT GCGGCGCTTC GAGAGGAAGG GCTTCAAGTT GGTGGCGCTG AAGCTGGTGC 241 AGGCCTCCGA GGAGCTGCTG CGTGAGCACT ACGCCGAGCT GCGTGAACGC CCGTTCTACG 301 GCCGCCTTGT CAAGTATATG GCCTCCGGGC CGGTGGTGGC CATGGTTTGG CAGGGGCTGG 361 ACGTGGTGCG CACCTCGCGG GCGCTCATCG GAGCCACGAA CCCGGCCGAC GCCCCGCCCG 421 GCACCATCCG CGGGGATTTC TGCATCGAGG TTGGCAAGAA CCTGATTCAC GGCAGCGACT 481 CGGTGGAGAG TGCCCGCCGC GAGATCGCTC TCTGGTTCCG CGCAGACGAG CTCCTCTGCT 541 GGGAGGACAG CGCTGGGCAC TGGCTGTATG AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 721 ATTGTACTGA ACGTTCCGTG GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt