Construct: ORF TRCN0000480574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002266.1_s317c1
- Derived from:
- ccsbBroadEn_01102
- DNA Barcode:
- GTGTTGCATTATTGCGTATGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMT1 (4836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480574
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4836 | NMT1 | N-myristoyltransferase 1 | NM_021079.5 | 99.9% | 100% | 487C>T |
| 2 | human | 4836 | NMT1 | N-myristoyltransferase 1 | XR_002958013.1 | 25.3% | (many diffs) | |
| 3 | mouse | 18107 | Nmt1 | N-myristoyltransferase 1 | NM_008707.3 | 90.9% | 97.3% | (many diffs) |
| 4 | mouse | 18107 | Nmt1 | N-myristoyltransferase 1 | XM_006532445.3 | 90.9% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacgag agtgagacag cagtgaagcc gccggcacct ccgctgccgc 121 agatgatgga agggaacggg aacggccatg agcactgcag cgattgcgag aatgaggagg 181 acaacagcta caaccggggt ggtttgagtc cagccaatga cactggagcc aaaaagaaga 241 aaaagaaaca aaaaaagaag aaagaaaaag gcagtgagac agattcagcc caggatcagc 301 ctgtgaagat gaactctttg ccagcagaga ggatccagga aatacagaag gccattgagc 361 tgttctcagt gggtcaggga cctgccaaaa ccatggagga ggctagcaag cgaagctacc 421 agttctggga tacgcagccc gtccccaagc tgggcgaagt ggtgaacacc catggccccg 481 tggagcctga caaggacaat atccgccagg agccctacac cctgccccag ggcttcacct 541 gggatgcttt ggacttgggc gatcgtggtg tgctaaaaga actgtacacc ctcctgaatg 601 agaactatgt ggaagatgat gacaacatgt tccgatttga ttattccccg gagtttcttt 661 tgtgggctct ccggccaccc ggctggctcc cccagtggca ctgtggggtt cgagtggtct 721 caagtcggaa attggttggg ttcattagcg ccatcccagc aaacatccat atctatgaca 781 cagagaagaa gatggtagag atcaacttcc tgtgtgtcca caagaagctg cgttccaaga 841 gggttgctcc agttctgatc cgagagatca ccaggcgggt tcacctggag ggcatcttcc 901 aagcagttta cactgccggg gtggtactac caaagcccgt tggcacctgc aggtattggc 961 atcggtccct aaacccacgg aagctgattg aagtgaagtt ctcccacctg agcagaaata 1021 tgaccatgca gcgcaccatg aagctctacc gactgccaga gactcccaag acagctgggc 1081 tgcgaccaat ggaaacaaag gacattccag tagtgcacca gctcctcacc aggtacttga 1141 agcaatttca CCTTACGCCC GTCATGAGCC AGGAGGAGGT GGAGCACTGG TTCTACCCCC 1201 AGGAGAATAT CATCGACACT TTCGTGGTGG AGAACGCAAA CGGAGAGGTG ACAGATTTCC 1261 TGAGCTTTTA TACGCTGCCC TCCACCATCA TGAACCATCC AACCCACAAG AGTCTCAAAG 1321 CTGCTTATTC TTTCTACAAC GTTCACACCC AGACCCCTCT TCTAGACCTC ATGAGCGACG 1381 CCCTTGTCCT CGCCAAAATG AAAGGGTTTG ATGTGTTCAA TGCACTGGAT CTCATGGAGA 1441 ACAAAACCTT CCTGGAGAAG CTCAAGTTTG GCATAGGGGA CGGCAACCTG CAGTATTACC 1501 TTTACAATTG GAAATGCCCC AGCATGGGGG CAGAGAAGGT TGGACTGGTG CTACAATTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAGTGTTGCA TTATTGCGTA TGTCGGACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt