Transcript: Human XR_002958013.1

PREDICTED: Homo sapiens N-myristoyltransferase 1 (NMT1), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NMT1 (4836)
Length:
5860
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002958013.1
NBCI Gene record:
NMT1 (4836)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002958013.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035709 CCAGCAAACATCCATATCTAT pLKO.1 698 3UTR 100% 5.625 3.938 N NMT1 n/a
2 TRCN0000307144 CCAGCAAACATCCATATCTAT pLKO_005 698 3UTR 100% 5.625 3.938 N NMT1 n/a
3 TRCN0000035712 CGGAAATTGGTTGGGTTCATT pLKO.1 668 3UTR 100% 5.625 3.938 N NMT1 n/a
4 TRCN0000289868 CGGAAATTGGTTGGGTTCATT pLKO_005 668 3UTR 100% 5.625 3.938 N NMT1 n/a
5 TRCN0000035710 CCAACCCACAAGAGTCTCAAA pLKO.1 1241 3UTR 100% 4.950 3.465 N NMT1 n/a
6 TRCN0000289869 CCAACCCACAAGAGTCTCAAA pLKO_005 1241 3UTR 100% 4.950 3.465 N NMT1 n/a
7 TRCN0000055167 CAGGAAATACAGAAGGCCATT pLKO.1 278 3UTR 100% 4.050 2.835 N Nmt1 n/a
8 TRCN0000301517 CAGGAAATACAGAAGGCCATT pLKO_005 278 3UTR 100% 4.050 2.835 N Nmt1 n/a
9 TRCN0000035713 CCTGAGCAGAAATATGACCAT pLKO.1 949 3UTR 100% 2.640 1.848 N NMT1 n/a
10 TRCN0000289867 CCTGAGCAGAAATATGACCAT pLKO_005 949 3UTR 100% 2.640 1.848 N NMT1 n/a
11 TRCN0000035711 CCAGGAAATACAGAAGGCCAT pLKO.1 277 3UTR 100% 2.160 1.512 N NMT1 n/a
12 TRCN0000289870 CCAGGAAATACAGAAGGCCAT pLKO_005 277 3UTR 100% 2.160 1.512 N NMT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002958013.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01102 pDONR223 100% 25.3% None (many diffs) n/a
2 ccsbBroad304_01102 pLX_304 0% 25.3% V5 (many diffs) n/a
3 TRCN0000480574 GTGTTGCATTATTGCGTATGTCGG pLX_317 27.5% 25.3% V5 (many diffs) n/a
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