Construct: ORF TRCN0000480580

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017960.1_s317c1
Derived from:
ccsbBroadEn_06812
DNA Barcode:
AGTTTTACACCGCCACACTGTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTH1R (5745)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480580
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5745 PTH1R parathyroid hormone 1 receptor NM_000316.3 99.9% 100% 1389T>C
2 human 5745 PTH1R parathyroid hormone 1 receptor NM_001184744.1 99.9% 100% 1389T>C
3 human 5745 PTH1R parathyroid hormone 1 receptor XM_017006933.1 99.9% 100% 1389T>C
4 human 5745 PTH1R parathyroid hormone 1 receptor XM_011533967.3 97.7% 97.8% 175_213del;1428T>C
5 human 5745 PTH1R parathyroid hormone 1 receptor XM_017006932.2 97.7% 97.8% 175_213del;1428T>C
6 human 5745 PTH1R parathyroid hormone 1 receptor XM_011533968.2 95% 92.5% (many diffs)
7 human 5745 PTH1R parathyroid hormone 1 receptor XM_005265344.3 94.7% 94.7% 0_1ins93;1296T>C
8 human 5745 PTH1R parathyroid hormone 1 receptor XM_017006934.1 82.9% 68.6% (many diffs)
9 mouse 19228 Pth1r parathyroid hormone 1 receptor NM_001083935.1 86.5% 90.2% (many diffs)
10 mouse 19228 Pth1r parathyroid hormone 1 receptor NM_001083936.1 86.5% 90.2% (many diffs)
11 mouse 19228 Pth1r parathyroid hormone 1 receptor NM_011199.2 86.5% 90.2% (many diffs)
12 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_006511988.3 84.2% 85.7% (many diffs)
13 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_017313215.1 84.2% 85.7% (many diffs)
14 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_006511987.2 82.8% 84.2% (many diffs)
15 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_017313214.1 78.8% 78.8% (many diffs)
16 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_006511985.2 69.7% 69.9% (many diffs)
17 mouse 19228 Pth1r parathyroid hormone 1 receptor XM_011242942.1 56% 55.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1848
ORF length:
1779
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggaccgcc cggatcgcac ccggcctggc gctcctgctc tgctgccccg
121tgctcagctc cgcgtacgcg ctggtggatg cagatgacgt catgactaaa gaggaacaga
181tcttcctgct gcaccgtgct caggcccagt gcgaaaaacg gctcaaggag gtcctgcaga
241ggccagccag cataatggaa tcagacaagg gatggacatc tgcgtccaca tcagggaagc
301ccaggaaaga taaggcatct gggaagctct accctgagtc tgaggaggac aaggaggcac
361ccactggcag caggtaccga gggcgcccct gtctgccgga atgggaccac atcctgtgct
421ggccgctggg ggcaccaggt gaggtggtgg ctgtgccctg tccggactac atttatgact
481tcaatcacaa aggccatgcc taccgacgct gtgaccgcaa tggcagctgg gagctggtgc
541ctgggcacaa caggacgtgg gccaactaca gcgagtgtgt caaatttctc accaatgaga
601ctcgtgaacg ggaggtgttt gaccgcctgg gcatgattta caccgtgggc tactccgtgt
661ccctggcgtc cctcaccgta gctgtgctca tcctggccta ctttaggcgg ctgcactgca
721cgcgcaacta catccacatg cacctgttcc tgtccttcat gctgcgcgcc gtgagcatct
781tcgtcaagga cgctgtgctc tactctggcg ccacgcttga tgaggctgag cgcctcaccg
841aggaggagct gcgcgccatc gcccaggcgc ccccgccgcc tgccaccgcc gctgccggct
901acgcgggctg cagggtggct gtgaccttct tcctttactt cctggccacc aactactact
961ggattctggt ggaggggctg tacctgcaca gcctcatctt catggccttc ttctcagaga
1021agaagtacct gtggggcttc acagtcttcg gctggggtct gcccgctgtc ttcgtggctg
1081tgtgggtcag tgtcagagct accctggcca acaccgggtg ctgggacttg agctccggga
1141acaaaaagtg gatcatccag gtgcccatcc tggcctccat tgtgctcaac ttcatcctct
1201tcatcaatat cgtccgggtg ctcgccacca agctgcggga gaccaacgcc ggccggtgtg
1261acacacggca gcagtaccgg aagctgctca aatccacgct ggtgctcatg cccctctttg
1321gcgtccacta cattgtcttc atggccacac catacaccga ggtctcaggg acgctctggc
1381aagtccagat gcactatgag atgctcttca actccttcca gggatttttt gtcgcaatca
1441tatactgttt ctgcaacggc gaggtacaag ctgagatcaa gaaatcttgG AGCCGCTGGA
1501CACTGGCACT GGACTTCAAG CGAAAGGCAC GCAGCGGGAG CAGCAGCTAT AGCTACGGCC
1561CCATGGTGTC CCACACAAGT GTGACCAATG TCGGCCCCCG TGTGGGACTC GGCCTGCCCC
1621TCAGCCCCCG CCTACTGCCC ACTGCCACCA CCAACGGCCA CCCTCAGCTG CCTGGCCATG
1681CCAAGCCAGG GACCCCAGCC CTGGAGACCC TCGAGACCAC ACCACCTGCC ATGGCTGCTC
1741CCAAGGACGA TGGGTTCCTC AACGGCTCCT GCTCAGGCCT GGACGAGGAG GCCTCTGGGC
1801CTGAGCGGCC ACCTGCCCTG CTACAGGAAG AGTGGGAGAC AGTCATGTTG CCAACTTTCT
1861TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1921CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1981GTGGAAAGGA CGAAGTTTTA CACCGCCACA CTGTTTCACG CGTTAAGTCg acaatcaacc
2041tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)