Construct: ORF TRCN0000480581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017573.1_s317c1
- Derived from:
- ccsbBroadEn_08432
- DNA Barcode:
- GAAATGCCGATACTTTTCTCATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SARS2 (54938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54938 | SARS2 | seryl-tRNA synthetase 2, mi... | NM_017827.4 | 99.9% | 100% | 1344C>T |
| 2 | human | 54938 | SARS2 | seryl-tRNA synthetase 2, mi... | NM_001145901.2 | 95.7% | 93.7% | (many diffs) |
| 3 | mouse | 71984 | Sars2 | seryl-aminoacyl-tRNA synthe... | NM_023637.3 | 85.3% | 86.1% | (many diffs) |
| 4 | mouse | 71984 | Sars2 | seryl-aminoacyl-tRNA synthe... | XM_006540376.2 | 43.4% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1620
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgtccatg gcgcggcgct tgtggccttt gctgactcgt cgggggttcc 121 ggccccgggg aggctgcatc tccaacgata gtccaaggag aagtttcact acagagaaac 181 gaaaccggaa cctcctgtac gagtatgcgc gcgagggcta cagcgcactc cctcagctgg 241 acatagagcg gttctgcgca tgcccagaag aggccgcaca cgccctggag ctccgcaagg 301 gggagctgcg ctcggcggac ctgcccgcga tcatctcgac atggcaggag ctgaggcagc 361 tgcaggagca gatccggagc ctggaggaag agaaggcagc tgtgactgag gcagtgcggg 421 ccctgctggc aaaccaggac agtggtgaag tgcagcagga ccccaagtac cagggtctgc 481 gggcacgtgg ccgggagatc cggaaggagc ttgttcacct gtaccccagg gaggcccagc 541 ttgaggagca gttctacctg caggcgctga agctgcccaa ccagacccac ccagacgtgc 601 ccgtcgggga tgagagccag gctcgagtgc tccacatggt cggagacaag ccagttttct 661 ccttccaacc tcggggccac ctggaaattg gcgagaaact cgacatcatc cgtcagaagc 721 gcctgtccca cgtgtctggc caccggtcct attacctgcg cggggctgga gccctcctgc 781 agcacggcct ggtcaacttc acattcaaca agcttctccg ccggggcttc acccccatga 841 cggtgccaga ccttctccgc ggagcagtgt ttgaaggctg tgggatgaca ccaaatgcca 901 acccatccca aatttacaac atcgaccctg cccgcttcaa agatctcaac ctggctggaa 961 cagcggaggt ggggcttgca ggctacttca tggaccacac cgtggccttc agggacctgc 1021 cagtcaggat ggtttgctcc agcacctgct accgggcaga gacaaacacg ggacaggaac 1081 cccgggggct gtatcgagta caccacttca ccaaggtgga gatgtttggg gtgacaggcc 1141 ctgggctgga gcagagctca cagctgctgg aggagttcct gtcccttcag atggagatct 1201 tgacagagct gggcttgcac ttccgggtcc tggatatgcc cacccaagaa ctgggcctcc 1261 ccgcctaccg caagtttgac attgaggcct ggatgccagg ccgaggccgc tttggagagg 1321 TCACCAGTGC TTCCAACTGC ACAGACTTCC AGAGCCGCCG CCTCCACATC ATGTTCCAGA 1381 CCGAGGCTGG GGAGCTGCAG TTTGCCCATA CGGTGAACGC CACCGCCTGT GCTGTCCCCC 1441 GCCTTCTCAT CGCGCTCCTG GAGAGTAACC AGCAGAAGGA CGGCTCAGTG CTCGTGCCCC 1501 CTGCCCTCCA GTCCTACCTC GGCACTGATC GGATCACAGC CCCTACCCAC GTGCCTCTCC 1561 AGTACATCGG CCCCAACCAG CCCCGGAAGC CTGGGCTGCC TGGCCAGCCT GCTGTAAGCT 1621 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGAGAAAT GCCGATACTT TTCTCATGGA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt