Construct: ORF TRCN0000480593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012461.2_s317c1
- Derived from:
- ccsbBroadEn_12133
- DNA Barcode:
- CCAACCCAGCCGAAGGGAAAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARPBP (55010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55010 | PARPBP | PARP1 binding protein | XM_011538515.1 | 81.1% | 80.9% | 1_204del;314G>C |
| 2 | human | 55010 | PARPBP | PARP1 binding protein | XM_024449028.1 | 81.1% | 80.9% | 1_204del;314G>C |
| 3 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019540.1 | 67.6% | 49% | (many diffs) |
| 4 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019539.1 | 66.4% | 48.6% | (many diffs) |
| 5 | human | 55010 | PARPBP | PARP1 binding protein | NM_001319993.1 | 58.9% | 58.8% | 1_612del;722G>C |
| 6 | human | 55010 | PARPBP | PARP1 binding protein | NM_001319994.1 | 58.9% | 58.8% | 1_612del;722G>C |
| 7 | human | 55010 | PARPBP | PARP1 binding protein | XM_011538514.2 | 58.9% | 58.8% | 1_612del;722G>C |
| 8 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019535.1 | 58.9% | 58.8% | 1_612del;722G>C |
| 9 | human | 55010 | PARPBP | PARP1 binding protein | XM_011538509.1 | 58.6% | 58.4% | 1_621del;731G>C |
| 10 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019537.2 | 57.1% | 40.9% | (many diffs) |
| 11 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019534.1 | 56.2% | 56.1% | 1_684del;794G>C |
| 12 | human | 55010 | PARPBP | PARP1 binding protein | NM_017915.4 | 50.7% | 50.6% | 1_855del;965G>C |
| 13 | human | 55010 | PARPBP | PARP1 binding protein | NM_001319988.1 | 44.7% | 44.6% | 1_1086del;1196G>C |
| 14 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019536.2 | 35.8% | 35.7% | 1_855del;965G>C;1004_1005ins258 |
| 15 | human | 55010 | PARPBP | PARP1 binding protein | XM_017019538.2 | 23.5% | 23.3% | (many diffs) |
| 16 | human | 55010 | PARPBP | PARP1 binding protein | XR_001748775.2 | 23.5% | (many diffs) | |
| 17 | human | 55010 | PARPBP | PARP1 binding protein | XR_001748776.2 | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 951
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaactgatt aaaggccaaa acagcaggga tcctttttgc aaagcaatag 121 aggaagttgc tcaggatttg gatttgagga ttaaaaatat tatcaattct caagaagctg 181 ttgtagctct tagcaccact gacatcagtc ctgctcggcc aaaatctcat gccataaacc 241 atggtactgc atactgtggc agagatactg tgaaagcctt attagttctt ttggacgaag 301 aagcagctaa tgctcctacc aaaaacaaag cagagctttt atatgatgag gaaaacacaa 361 tccatcatca tggaacgtct attcttacac tttttaggtc tcccacacag gtgaataatt 421 cgataaaacc cctaagagaa cgcatctgtg tgtcaatgca agagaaaaaa attaagatga 481 agcaaacttt aattagatcc caatttgctt gtacttataa agatgactac atgataagca 541 aggataattg gaataatgtt aatttagcat caaagccttt gtgtgttctt tacatggaaa 601 atgacctttc tgagggtgta aaTCCATCTG TTGGAAGATC AACAATTGGA ACGAGTTTTG 661 GAAATGTTCA TCTGGACAGA AGTAAAAATG AAAAAGTATC AAGAAAATCA ACCAGTCAGA 721 CAGGAAATAA AAGCTCAAAA AGGAAACAGG TGGATTTGGA TGGTGAAAAT ATTCTCTGTG 781 ATAATAGAAA TGAACCACCT CAACATAAAA ATGCTAAAAT ACCTAAGAAA TCAAATGATT 841 CACAGAATAG ATTGTACGGC AAACTAGCTA AAGTAGCAAA AAGTAATAAA TGTACTGCCA 901 AGGACAAGTT GATTTCTGGC CAGGCAAAGT TAACTCAGTT TTTTAGACTA TTGCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGACCAA CCCAGCCGAA GGGAAAAAAG ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt