Construct: ORF TRCN0000480657

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015858.1_s317c1
Derived from:
ccsbBroadEn_13921
DNA Barcode:
GTTCGGCTGCCTTTGCTGGACGTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PLXNA2 (5362)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480657
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5362 PLXNA2 plexin A2 NM_025179.4 99.9% 44.2% (many diffs)
2 human 5362 PLXNA2 plexin A2 XM_005273164.3 95.8% 42.4% (many diffs)
3 human 5362 PLXNA2 plexin A2 XM_005273165.4 88.1% 45.9% (many diffs)
4 human 5362 PLXNA2 plexin A2 XR_002956815.1 42.4% (many diffs)
5 mouse 18845 Plxna2 plexin A2 NM_008882.2 88.3% 41.9% (many diffs)
6 mouse 18845 Plxna2 plexin A2 XM_006497240.3 88% 41.7% (many diffs)
7 mouse 18845 Plxna2 plexin A2 XR_373891.3 46% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2802
ORF length:
2733
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaacagagg cggccctggc cccgggccct ggaggtggac agccgctctg
121tggtcctgct ctcagtggtc tgggtgctgc tggccccccc agcagccggc atgcctcagt
181tcagcacctt ccactctgag aatcgtgact ggaccttcaa ccacttgacc gtccaccaag
241ggacgggggc cgtctatgtg ggggccatca accgggtcta taagctgaca ggcaacctga
301ccatccaggt ggctcataag acagggccag aagaggacaa caagtcttgt tacccgcccc
361tcatcgtgca gccctgcagc gaagtgctca ccctcaccaa caatgtcaac aagctgctca
421tcattgacta ctctgagaac cgcctgctgg cctgtgggag cctctaccag ggggtctgca
481agctgctgcg gctggatgac ctcttcatcc tggtggagcc atcccacaag aaggagcact
541acctgtccag tgtcaacaag acgggcacca tgtacggggt gattgtgcgc tctgagggtg
601aggatggcaa gctcttcatc ggcacggctg tggatgggaa gcaggattac ttcccgaccc
661tgtccagccg gaagctgccc cgagaccctg agtcctcagc catgctcgac tatgagctac
721acagcgattt tgtctcctct ctcatcaaga tcccttcaga caccctggcc ctggtctccc
781actttgacat cttctacatc tacggctttg ctagtggggg ctttgtctac tttctcactg
841tccagcccga gacccctgag ggtgtggcca tcaactccgc tggagacctc ttctacacct
901cacgcatcgt gcggctctgc aaggatgacc ccaagttcca ctcatacgtg tccctgccct
961tcggctgcac ccgggccggg gtggaatacc gcctcctgca ggctgcttac ctggccaagc
1021ctggggactc actggcccag gccttcaata tcaccagcca ggacgatgta ctctttgcca
1081tcttctccaa agggcagaag cagtatcacc acccgcccga tgantctncc ctgtgtgcct
1141tccctatccg ggccatcaac ttgcagatca aggggcgcct acagtcctgc taccagggcg
1201agggcaacct ggagctcaac tggctgctgg ggaaggacgt ccagtgcacc aaggcgcctg
1261tccccatcga tgataacttc tgtggactgg acatcaacca gcccctggga ggctcaactc
1321cagtggaggg cctgaccctg tacaccacca gcagggaccg catgacctct gtggcctcct
1381acgtttacaa cggctacagc gtggtttttg tggggactaa gagtggcaag ctgaaaaaga
1441ttcgggccga cggtcccccc catggtgggg tccagtacga gatggtctct gtgctcaagg
1501acggaagccc catcctccgg gacatggcct tctccattga tcagcgctac ctgtacgtca
1561tgtctgagag acaggtcacc agggtccccg tggagtcatg tgagcagtat acgacttgtg
1621gggagtgcct gagctctggg gaccctcact gtggctggtg tgccctgcac aacatgtgct
1681cccgcaggga caaatgccaa caggcctggg aacctaatcg atttgctgcc agcatcagcc
1741agtgtgtgag ccttgcagtg catcccagca gcatctcagt atctgagcac agccggttgc
1801ttagcctggt agtgagtgat gctcctgatc tatctgcggg tatcgcctgt gcctttggga
1861acctgacaga ggtggagggg caggtgtccg ggagccaggt catctgcatc tcacctgggc
1921ccaaggatgt ccctgtcatc ccgctggatc aagactggtt tgggctggag ctacagctga
1981ggtccaagga gacagggaag atatttgtca gcaccgagtt caagttttac aactgcagtg
2041cccaccaact gtgcctgtcc tgtgtcaaca gcgccttccg ctgccattgg tgcaagtacc
2101gcaacctctg cactcatgac cccaccacct gctccttcca ggagggccgg atcaatattt
2161cagaggactg tccccagctg gtgcccacag aggagatctt gattccagtc ggggaggtaa
2221agccaatcac ccttaaggcg cgaaatctgc cccagccgca gtccggccag cgaggctatg
2281agtgtgtcct caacatacaa ggagccatcc accgggtccc cgctctgcgc ttcaacagct
2341ccagcgttca gtgtcagaac agctcgtacc agtatgatgg catggacatc agcaatctgg
2401ccgtggattt cgctgtggtg tggaacggca atttcatcat tgacaaccct caggacctga
2461aagtccatct ctacaagtgt gcagcccagc gggagagctg cggcctctgc ctcaaggccg
2521accggaagtt tgagtgtggc tggtgcagcg gcgagcgcag gtgcaccctc caccagcact
2581gtaccagccc ttccagcccc ctggctcgac tggtccagcc acaatgtcaa gtgctccaac
2641cctcaaatca ccgagatttt gacggtgtct ggaccgccgg aaggagggac gcgagtgacc
2701atccatggcg tgaacctggg tctggacttc tccgagatcg cccaccatgt gcaggtggct
2761ggggtgccct gcacgcccct cccaggggaa tacatcatcg ctgagcagat tgtctgtgag
2821atgggccatg ccctcgtggg aaccacctcc gggccagtac gcctgtgtat tggcgagtgt
2881aagccagagt tcatgacgaa gtcccatcag cagtacacct tcgtgaaccc ttctgtgctg
2941tcactcaacc caatccgagg tcccgagtca ggaggcacta tggtgaccat taccggccat
3001taccttgggg ctgggagcag cgtggcagtc tacctgggca accagacctg cgagttctac
3061gggaggtcaa tgagtgagat cgtgtgtgtc tcacccccat catccaatgg ccttggcccg
3121gtccctgttt ctgtgagtgt cgaccgagcc catgtggata gcaacctgca gtttgagtac
3181atagatgacc ctcgggtcca gcgcatcgag ccagagtgga gcattgccag tggccacaca
3241cccctgacca tcacaggctt caacctggat gtcattcagg agccaaggat ccgagtcaaa
3301ttcaatggca aagaatctgt caatgtgtgt aaagttgtga acacaaccac cctcacctgc
3361ctggcaccct ctctgaccac ggactaccgc cctggcctgg acactgtgga acgcccagat
3421gagtttggat ttgtctttaa caatgtccaa tccttgctaa tttacaacga caccaagttt
3481atctactacc ccaacccgac ctttgaactg cttagcccta ctggagtctt ggatcaaaag
3541ccaggatcgc ccatcattct gaagggcaaa aacctctgcc ctcctgcctc tggaggggcc
3601aaactcaact acactgtgct catcggagag accccttgtg ctgtcaccgt atctgagacc
3661cagcttctct gcgagcctcc caacctcacc gggcagcaca aggtcatggt tcacgtgggc
3721gggatggtgt tctcgcctgg ctcggtgagt gtcatctcag acagcttgct gaccctgcca
3781gccatcgtca gcatcgcggc cggcggcagc ctcctcctca tcatcgtcat catcgtcctc
3841attgcctaca agcgcaagtc tcgagaaaat gacctcactc tcaagcggct gcaaatgcag
3901atggacaatc tggagtcccg tgtggccttg gagtgcaagg aagcttttgc tgagctccag
3961acggatatca atgagttgac cagtgacctg gaccgctcag gaatccctta cctggactat
4021cgtacctacg ctatgcgagt cctgttcccg ggcatcgagg accaccccgt cctgcgggag
4081ctggaggtac aaggaaacgg gcagcagcac gtggagaagg ccctgaagct ctttgcccag
4141ctcatcaaca acaaggtgtt cctgctgacc ttcatccgca ccctggagct gcagcgcagt
4201ttctccatgc gcgaccgggg caacgtggct tcgctcatca tgaccggcct gcagggccgc
4261ctggaatatg ccactgatgt cctcaagcag ctgctctctg acctcatcga taagaacctg
4321gagaacaaga accaccccaa gctgctactc cggaggacag agtctgtggc tgaaaagatg
4381ctgaccaatt ggttcgcctt cctcctgcac aagttcctaa aggagtgcgc aggggagcca
4441ctcttcatgc tatactgtgc catcaagcag cagatggaga agggccccat tgatgccatc
4501acgggcgagg cccgctactc cctgagcgag gacaagctca tccggcagca gatcgagtac
4561aagaccctga tcctgaactg cgtcaaccct gacaacgaga acagtccaga gatcccagtg
4621aaggtgttaa actgtgacac catcacacag gtcaaggaga agattcttga tgccgtgtat
4681aagaatgtgc cctattccca gcggccgagg gcagtggaca tggacttgga gtggcgccaa
4741ggccggatcg cccgggtcgt gctgcaagat gaggacatca ccaccaagat tgagggtgac
4801tggaagcggc tcaacacact gatgcattat caggtgtcag acaggtcggt ggtggctctg
4861gtccccaaac agacctcctc ctacaacatc cctgcctctg ccagcatctc ccggacgtcc
4921atcagcagat acgactcctc cttcaggtat acgggcagcc ccgacagcct gcggtcccgg
4981gccccgatga tcaccccaga cctggaaagt ggggtcaagg tgtggcatct ggtgaagaac
5041catgaccacg gtgaccagaa ggagggtgac cggggcagca agatggtgtc cgagatctac
5101ctgacccggc tactggccac caagggcacc ctgcagaagt ttgtggacga cttgtttgag
5161accttgttca gcactgtgca ccggggcagc gctctccccc tggccatcaa gtacatgttt
5221gatttcctag atgagcaggc agacaggcac agcatccatg acacagatgt gcggcacacc
5281tggaaaagca actgcctccc tctgcgcttc tgggtgaacg tgattaagaa cccccagttc
5341gtgtttgaca tccacaaggg cagcaTCACG GACGCCTGCC TCTCTGTGGT GGCCCAGACC
5401TTCATGGACT CTTGTTCAAC GTCAGAGCAC CGGCTGGGCA AGGACTCCCC CTCCAACAAG
5461CTGCTCTATG CCAAGGACAT CCCCAGCTAC AAGAGCTGGG TGGAGAGATA CTACGCAGAC
5521ATCGCCAAGC TCCCAGCCAT CAGTGACCAG GACATGAATG CCTACCTCGC CGAGCAGTCC
5581CGCCTGCACG CCGTGGAGTT CAACATGCTG AGTGCCCTCA ATGAGATCTA CTCCTATGTC
5641AGCAAGTATA GTGAGGAGCT CATCGGGGCC CTAGAGCAGG ATGAGCAGGC ACGGCGGCAG
5701CGGCTGGCTT ATAAGGTGGA GCAGCTCATT AATGCCATGT CCATTGAGAG CTTGCCAACT
5761TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT
5821TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGATTTC GATTTCTTGG CTTTATATAT
5881CTTGTGGAAA GGACGAGTTC GGCTGCCTTT GCTGGACGTC ACGCGTTAAG TCgacaatca
5941acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)