Construct: ORF TRCN0000480657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015858.1_s317c1
- Derived from:
- ccsbBroadEn_13921
- DNA Barcode:
- GTTCGGCTGCCTTTGCTGGACGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PLXNA2 (5362)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5362 | PLXNA2 | plexin A2 | NM_025179.4 | 99.9% | 44.2% | (many diffs) |
| 2 | human | 5362 | PLXNA2 | plexin A2 | XM_005273164.3 | 95.8% | 42.4% | (many diffs) |
| 3 | human | 5362 | PLXNA2 | plexin A2 | XM_005273165.4 | 88.1% | 45.9% | (many diffs) |
| 4 | human | 5362 | PLXNA2 | plexin A2 | XR_002956815.1 | 42.4% | (many diffs) | |
| 5 | mouse | 18845 | Plxna2 | plexin A2 | NM_008882.2 | 88.3% | 41.9% | (many diffs) |
| 6 | mouse | 18845 | Plxna2 | plexin A2 | XM_006497240.3 | 88% | 41.7% | (many diffs) |
| 7 | mouse | 18845 | Plxna2 | plexin A2 | XR_373891.3 | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2802
- ORF length:
- 2733
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacagagg cggccctggc cccgggccct ggaggtggac agccgctctg 121 tggtcctgct ctcagtggtc tgggtgctgc tggccccccc agcagccggc atgcctcagt 181 tcagcacctt ccactctgag aatcgtgact ggaccttcaa ccacttgacc gtccaccaag 241 ggacgggggc cgtctatgtg ggggccatca accgggtcta taagctgaca ggcaacctga 301 ccatccaggt ggctcataag acagggccag aagaggacaa caagtcttgt tacccgcccc 361 tcatcgtgca gccctgcagc gaagtgctca ccctcaccaa caatgtcaac aagctgctca 421 tcattgacta ctctgagaac cgcctgctgg cctgtgggag cctctaccag ggggtctgca 481 agctgctgcg gctggatgac ctcttcatcc tggtggagcc atcccacaag aaggagcact 541 acctgtccag tgtcaacaag acgggcacca tgtacggggt gattgtgcgc tctgagggtg 601 aggatggcaa gctcttcatc ggcacggctg tggatgggaa gcaggattac ttcccgaccc 661 tgtccagccg gaagctgccc cgagaccctg agtcctcagc catgctcgac tatgagctac 721 acagcgattt tgtctcctct ctcatcaaga tcccttcaga caccctggcc ctggtctccc 781 actttgacat cttctacatc tacggctttg ctagtggggg ctttgtctac tttctcactg 841 tccagcccga gacccctgag ggtgtggcca tcaactccgc tggagacctc ttctacacct 901 cacgcatcgt gcggctctgc aaggatgacc ccaagttcca ctcatacgtg tccctgccct 961 tcggctgcac ccgggccggg gtggaatacc gcctcctgca ggctgcttac ctggccaagc 1021 ctggggactc actggcccag gccttcaata tcaccagcca ggacgatgta ctctttgcca 1081 tcttctccaa agggcagaag cagtatcacc acccgcccga tgantctncc ctgtgtgcct 1141 tccctatccg ggccatcaac ttgcagatca aggggcgcct acagtcctgc taccagggcg 1201 agggcaacct ggagctcaac tggctgctgg ggaaggacgt ccagtgcacc aaggcgcctg 1261 tccccatcga tgataacttc tgtggactgg acatcaacca gcccctggga ggctcaactc 1321 cagtggaggg cctgaccctg tacaccacca gcagggaccg catgacctct gtggcctcct 1381 acgtttacaa cggctacagc gtggtttttg tggggactaa gagtggcaag ctgaaaaaga 1441 ttcgggccga cggtcccccc catggtgggg tccagtacga gatggtctct gtgctcaagg 1501 acggaagccc catcctccgg gacatggcct tctccattga tcagcgctac ctgtacgtca 1561 tgtctgagag acaggtcacc agggtccccg tggagtcatg tgagcagtat acgacttgtg 1621 gggagtgcct gagctctggg gaccctcact gtggctggtg tgccctgcac aacatgtgct 1681 cccgcaggga caaatgccaa caggcctggg aacctaatcg atttgctgcc agcatcagcc 1741 agtgtgtgag ccttgcagtg catcccagca gcatctcagt atctgagcac agccggttgc 1801 ttagcctggt agtgagtgat gctcctgatc tatctgcggg tatcgcctgt gcctttggga 1861 acctgacaga ggtggagggg caggtgtccg ggagccaggt catctgcatc tcacctgggc 1921 ccaaggatgt ccctgtcatc ccgctggatc aagactggtt tgggctggag ctacagctga 1981 ggtccaagga gacagggaag atatttgtca gcaccgagtt caagttttac aactgcagtg 2041 cccaccaact gtgcctgtcc tgtgtcaaca gcgccttccg ctgccattgg tgcaagtacc 2101 gcaacctctg cactcatgac cccaccacct gctccttcca ggagggccgg atcaatattt 2161 cagaggactg tccccagctg gtgcccacag aggagatctt gattccagtc ggggaggtaa 2221 agccaatcac ccttaaggcg cgaaatctgc cccagccgca gtccggccag cgaggctatg 2281 agtgtgtcct caacatacaa ggagccatcc accgggtccc cgctctgcgc ttcaacagct 2341 ccagcgttca gtgtcagaac agctcgtacc agtatgatgg catggacatc agcaatctgg 2401 ccgtggattt cgctgtggtg tggaacggca atttcatcat tgacaaccct caggacctga 2461 aagtccatct ctacaagtgt gcagcccagc gggagagctg cggcctctgc ctcaaggccg 2521 accggaagtt tgagtgtggc tggtgcagcg gcgagcgcag gtgcaccctc caccagcact 2581 gtaccagccc ttccagcccc ctggctcgac tggtccagcc acaatgtcaa gtgctccaac 2641 cctcaaatca ccgagatttt gacggtgtct ggaccgccgg aaggagggac gcgagtgacc 2701 atccatggcg tgaacctggg tctggacttc tccgagatcg cccaccatgt gcaggtggct 2761 ggggtgccct gcacgcccct cccaggggaa tacatcatcg ctgagcagat tgtctgtgag 2821 atgggccatg ccctcgtggg aaccacctcc gggccagtac gcctgtgtat tggcgagtgt 2881 aagccagagt tcatgacgaa gtcccatcag cagtacacct tcgtgaaccc ttctgtgctg 2941 tcactcaacc caatccgagg tcccgagtca ggaggcacta tggtgaccat taccggccat 3001 taccttgggg ctgggagcag cgtggcagtc tacctgggca accagacctg cgagttctac 3061 gggaggtcaa tgagtgagat cgtgtgtgtc tcacccccat catccaatgg ccttggcccg 3121 gtccctgttt ctgtgagtgt cgaccgagcc catgtggata gcaacctgca gtttgagtac 3181 atagatgacc ctcgggtcca gcgcatcgag ccagagtgga gcattgccag tggccacaca 3241 cccctgacca tcacaggctt caacctggat gtcattcagg agccaaggat ccgagtcaaa 3301 ttcaatggca aagaatctgt caatgtgtgt aaagttgtga acacaaccac cctcacctgc 3361 ctggcaccct ctctgaccac ggactaccgc cctggcctgg acactgtgga acgcccagat 3421 gagtttggat ttgtctttaa caatgtccaa tccttgctaa tttacaacga caccaagttt 3481 atctactacc ccaacccgac ctttgaactg cttagcccta ctggagtctt ggatcaaaag 3541 ccaggatcgc ccatcattct gaagggcaaa aacctctgcc ctcctgcctc tggaggggcc 3601 aaactcaact acactgtgct catcggagag accccttgtg ctgtcaccgt atctgagacc 3661 cagcttctct gcgagcctcc caacctcacc gggcagcaca aggtcatggt tcacgtgggc 3721 gggatggtgt tctcgcctgg ctcggtgagt gtcatctcag acagcttgct gaccctgcca 3781 gccatcgtca gcatcgcggc cggcggcagc ctcctcctca tcatcgtcat catcgtcctc 3841 attgcctaca agcgcaagtc tcgagaaaat gacctcactc tcaagcggct gcaaatgcag 3901 atggacaatc tggagtcccg tgtggccttg gagtgcaagg aagcttttgc tgagctccag 3961 acggatatca atgagttgac cagtgacctg gaccgctcag gaatccctta cctggactat 4021 cgtacctacg ctatgcgagt cctgttcccg ggcatcgagg accaccccgt cctgcgggag 4081 ctggaggtac aaggaaacgg gcagcagcac gtggagaagg ccctgaagct ctttgcccag 4141 ctcatcaaca acaaggtgtt cctgctgacc ttcatccgca ccctggagct gcagcgcagt 4201 ttctccatgc gcgaccgggg caacgtggct tcgctcatca tgaccggcct gcagggccgc 4261 ctggaatatg ccactgatgt cctcaagcag ctgctctctg acctcatcga taagaacctg 4321 gagaacaaga accaccccaa gctgctactc cggaggacag agtctgtggc tgaaaagatg 4381 ctgaccaatt ggttcgcctt cctcctgcac aagttcctaa aggagtgcgc aggggagcca 4441 ctcttcatgc tatactgtgc catcaagcag cagatggaga agggccccat tgatgccatc 4501 acgggcgagg cccgctactc cctgagcgag gacaagctca tccggcagca gatcgagtac 4561 aagaccctga tcctgaactg cgtcaaccct gacaacgaga acagtccaga gatcccagtg 4621 aaggtgttaa actgtgacac catcacacag gtcaaggaga agattcttga tgccgtgtat 4681 aagaatgtgc cctattccca gcggccgagg gcagtggaca tggacttgga gtggcgccaa 4741 ggccggatcg cccgggtcgt gctgcaagat gaggacatca ccaccaagat tgagggtgac 4801 tggaagcggc tcaacacact gatgcattat caggtgtcag acaggtcggt ggtggctctg 4861 gtccccaaac agacctcctc ctacaacatc cctgcctctg ccagcatctc ccggacgtcc 4921 atcagcagat acgactcctc cttcaggtat acgggcagcc ccgacagcct gcggtcccgg 4981 gccccgatga tcaccccaga cctggaaagt ggggtcaagg tgtggcatct ggtgaagaac 5041 catgaccacg gtgaccagaa ggagggtgac cggggcagca agatggtgtc cgagatctac 5101 ctgacccggc tactggccac caagggcacc ctgcagaagt ttgtggacga cttgtttgag 5161 accttgttca gcactgtgca ccggggcagc gctctccccc tggccatcaa gtacatgttt 5221 gatttcctag atgagcaggc agacaggcac agcatccatg acacagatgt gcggcacacc 5281 tggaaaagca actgcctccc tctgcgcttc tgggtgaacg tgattaagaa cccccagttc 5341 gtgtttgaca tccacaaggg cagcaTCACG GACGCCTGCC TCTCTGTGGT GGCCCAGACC 5401 TTCATGGACT CTTGTTCAAC GTCAGAGCAC CGGCTGGGCA AGGACTCCCC CTCCAACAAG 5461 CTGCTCTATG CCAAGGACAT CCCCAGCTAC AAGAGCTGGG TGGAGAGATA CTACGCAGAC 5521 ATCGCCAAGC TCCCAGCCAT CAGTGACCAG GACATGAATG CCTACCTCGC CGAGCAGTCC 5581 CGCCTGCACG CCGTGGAGTT CAACATGCTG AGTGCCCTCA ATGAGATCTA CTCCTATGTC 5641 AGCAAGTATA GTGAGGAGCT CATCGGGGCC CTAGAGCAGG ATGAGCAGGC ACGGCGGCAG 5701 CGGCTGGCTT ATAAGGTGGA GCAGCTCATT AATGCCATGT CCATTGAGAG CTTGCCAACT 5761 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 5821 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGATTTC GATTTCTTGG CTTTATATAT 5881 CTTGTGGAAA GGACGAGTTC GGCTGCCTTT GCTGGACGTC ACGCGTTAAG TCgacaatca 5941 acctctggat tacaaaattt gtgaaagatt