Transcript: Mouse XM_006497240.3

PREDICTED: Mus musculus plexin A2 (Plxna2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plxna2 (18845)
Length:
10896
CDS:
642..6347

Additional Resources:

NCBI RefSeq record:
XM_006497240.3
NBCI Gene record:
Plxna2 (18845)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006497240.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000328491 AGTCTGCAGTTCGAGTATATA pLKO_005 3735 CDS 100% 15.000 21.000 N Plxna2 n/a
2 TRCN0000328553 GCCTGTCCCAATCGATGATAA pLKO_005 1829 CDS 100% 13.200 18.480 N Plxna2 n/a
3 TRCN0000328488 CTCAACGAGATCTACTCATAT pLKO_005 6210 CDS 100% 13.200 10.560 N Plxna2 n/a
4 TRCN0000328552 AGATCCTCGATGCCGTATATA pLKO_005 5233 CDS 100% 15.000 10.500 N Plxna2 n/a
5 TRCN0000328487 CTATTCCCTTCTTAGTAATAT pLKO_005 6795 3UTR 100% 15.000 10.500 N Plxna2 n/a
6 TRCN0000078966 CGGACATCCATTAGCAGATAT pLKO.1 5505 CDS 100% 13.200 9.240 N Plxna2 n/a
7 TRCN0000078964 CGGCCAGATGAGTTTGGATTT pLKO.1 3984 CDS 100% 10.800 7.560 N Plxna2 n/a
8 TRCN0000078965 CCAGCCACAATGTCAAGTGTT pLKO.1 3187 CDS 100% 4.950 3.465 N Plxna2 n/a
9 TRCN0000078967 CCAGCCATTAGTGACCAAGAT pLKO.1 6126 CDS 100% 4.950 3.465 N Plxna2 n/a
10 TRCN0000078963 GCTATCACACTGGCTTCTCAA pLKO.1 6387 3UTR 100% 4.950 3.465 N Plxna2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006497240.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13921 pDONR223 100% 88% 41.7% None (many diffs) n/a
2 ccsbBroad304_13921 pLX_304 0% 88% 41.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000480657 GTTCGGCTGCCTTTGCTGGACGTC pLX_317 6.9% 88% 41.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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