Construct: ORF TRCN0000480667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008806.2_s317c1
- Derived from:
- ccsbBroadEn_09545
- DNA Barcode:
- TTTCAAGGATACTTACTATTGCAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAM1L1 (133022)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133022 | TRAM1L1 | translocation associated me... | NM_152402.3 | 99.8% | 99.7% | 176T>C;732A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1173
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctccgtaag aagagcacca agaacccccc cgttctcagc caggaattca 121 tcctgcagaa tcatgcggac atcgtctcct gcgtggggat gttcttcctg ctggggcttg 181 tgttcgaggg aacagcagaa gcatccatcg tgtttctcac tcttcagcac agtgttgctg 241 cccctgcagc agaggaacaa gccacgggct caaagtccct ctattattat ggtgtcaaag 301 atttggccac ggttttcttc tacatgctgg tggcaatcat tattcatgcc acaattcagg 361 aatatgtgtt ggataaaatt aacaagagaa tgcagttcac caaagcgaaa caaaacaagt 421 ttaacgagtc tggtcagttt agtgtgttct actttttttc ttgtatttgg ggcacattca 481 ttttaatctc tgaaaactgc ctgtcagacc caactcttat atggaaggct cgtccccata 541 gcatgatgac atttcaaatg aagtttttct acatatccca gttggcttac tggtttcatg 601 cttttcctga actctacttc cagaaaacca aaaaacaaga catccctcgt caacttgtct 661 acattggtct tcacctcttc caCATTACTG GAGCTTATCT CTTGTACTTG AATCATTTGG 721 GACTTCTTCT TTTGGTACTG CATTATTTTG TTGAATTACT TTCCCACATG TGCGGCCTGT 781 TTTACTTTAG TGATGAGAAG TACCAGAAAG GCATATCTCT GTGGGCCATT GTGTTTATCT 841 TGGGTAGACT TGTGACTTTA ATTGTTTCCG TACTCACTGT TGGGTTTCAC CTGGCTGGAT 901 CGCAGAATCG GAATCCTGAT GCCCTTACTG GAAATGTAAA TGTGTTGGCA GCTAAAATTG 961 CTGTTCTGTC GTCCAGTTGC ACGATCCAAG CCTACGTAAC ATGGAACTTA ATTACTCTCT 1021 GGCTTCAGAG GTGGGTAGAA GATTCTAATA TTCAGGCCTC ATGTATGAAA AAGAAACGGT 1081 CGAGATCTTC TAAAAAAAGA ACAGAAAACG GAGTGGGAGT GGAAACTTCA AATAGAGTAG 1141 ACTGTCCGCC AAAGAGGAAA GAGAAATCTT CATACCCACT TTCTTGTACA AAGTGGTTGA 1201 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1261 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT 1321 CAAGGATACT TACTATTGCA CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1381 tgtgaaagat t