Transcript: Human NM_152402.3

Homo sapiens translocation associated membrane protein 1 like 1 (TRAM1L1), mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
TRAM1L1 (133022)
Length:
2023
CDS:
184..1293

Additional Resources:

NCBI RefSeq record:
NM_152402.3
NBCI Gene record:
TRAM1L1 (133022)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152402.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000133678 GCTCAAAGTCCCTCTATTATT pLKO.1 386 CDS 100% 15.000 10.500 N TRAM1L1 n/a
2 TRCN0000136029 GAGGTGGGTAGAAGATTCTAA pLKO.1 1146 CDS 100% 5.625 3.938 N TRAM1L1 n/a
3 TRCN0000136126 GATGCCCTTACTGGAAATGTA pLKO.1 1036 CDS 100% 5.625 3.938 N TRAM1L1 n/a
4 TRCN0000135755 CTGCAAAGGAATCTGCTCTTT pLKO.1 1319 3UTR 100% 4.950 3.465 N TRAM1L1 n/a
5 TRCN0000136205 GTTTAACGAGTCTGGTCAGTT pLKO.1 537 CDS 100% 4.950 3.465 N TRAM1L1 n/a
6 TRCN0000134313 GTTTCTTTCTGCACTAGAGAT pLKO.1 1343 3UTR 100% 4.950 3.465 N TRAM1L1 n/a
7 TRCN0000137963 GCATCCATCGTGTTTCTCACT pLKO.1 319 CDS 100% 2.640 1.848 N TRAM1L1 n/a
8 TRCN0000137861 GTCAAAGATTTGGCCACGGTT pLKO.1 412 CDS 100% 2.640 1.848 N TRAM1L1 n/a
9 TRCN0000136108 CCAACTCTTATATGGAAGGCT pLKO.1 628 CDS 100% 0.750 0.525 N TRAM1L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152402.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09545 pDONR223 100% 99.8% 99.7% None 176T>C;732A>G n/a
2 ccsbBroad304_09545 pLX_304 0% 99.8% 99.7% V5 (not translated due to frame shift) 176T>C;732A>G n/a
3 TRCN0000480667 TTTCAAGGATACTTACTATTGCAC pLX_317 44.4% 99.8% 99.7% V5 (not translated due to frame shift) 176T>C;732A>G n/a
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