Construct: ORF TRCN0000480688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018277.1_s317c1
- Derived from:
- ccsbBroadEn_02311
- DNA Barcode:
- TCGTCTGCCGGACCTCGGCGTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARPC3 (10094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10094 | ARPC3 | actin related protein 2/3 c... | NM_001278556.2 | 100% | 100% | |
| 2 | human | 10094 | ARPC3 | actin related protein 2/3 c... | NM_001287222.1 | 99.4% | 99.4% | 378_379insGAA |
| 3 | mouse | 56378 | Arpc3 | actin related protein 2/3 c... | NM_019824.4 | 86.5% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggcttaccac tcttctctca tggatcctga taccaaactc atcggaaaca 121 tggcactgtt gcctatcaga agtcaattca aaggacctgc ccccagagag acaaaagata 181 cagatattgt ggatgaagcc atctattact tcaaggccaa tgtcTTCTTC AAAAACTATG 241 AAATTAAGAA TGAAGCTGAT AGGACCTTGA TATATATAAC TCTCTACATT TCTGAATGTC 301 TGAAGAAACT GCAAAAGTGC AATTCCAAAA GCCAAGGTGA GAAAGAAATG TATACGCTGG 361 GAATCACTAA TTTTCCCATT CCTGGAGAGC CTGGTTTTCC ACTTAACGCA ATTTATGCCA 421 AACCTGCAAA CAAACAGGAA GATGAAGTGA TGAGAGCCTA TTTACAACAG CTAAGGCAAG 481 AGACTGGACT GAGACTTTGT GAGAAAGTTT TCGACCCTCA GAATGATAAA CCCAGCAAGT 541 GGTGGACTTG CTTTGTGAAG AGACAGTTCA TGAACAAGAG TCTTTCAGGA CCTGGACAGT 601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGATCGTC TGCCGGACCT CGGCGTGAAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt