Construct: ORF TRCN0000480706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005058.1_s317c1
- Derived from:
- ccsbBroadEn_11857
- DNA Barcode:
- TAAGGGACCGCTCACGGTCCCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF16 (27237)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XM_024446454.1 | 99.7% | 99.7% | 244C>T;627T>C;813G>A |
| 2 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XM_024446455.1 | 99.7% | 99.7% | 244C>T;627T>C;813G>A |
| 3 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | NM_014448.4 | 59.2% | 59.2% | (many diffs) |
| 4 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XM_017001050.1 | 59.2% | 59.2% | (many diffs) |
| 5 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XM_017001051.1 | 59.2% | 59.2% | (many diffs) |
| 6 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XM_017001049.1 | 57.8% | 57.8% | (many diffs) |
| 7 | human | 27237 | ARHGEF16 | Rho guanine nucleotide exch... | XR_001737117.1 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgagatcctc acgtcggagt tctcctacca gcacagcctg agcatcctgg 121 tggaggagtt cctgcagtcc aaggagctgc gggcgaccgt gacccagatg gagcaccacc 181 acctcttctc caacatcctg gatgtcctgg gtgccagtca gaggttcttc gaggacctgg 241 agcagcggca caaggcccag gtgctggtcg aggacatcag tgacatcctg gaggagcacg 301 ctgagaagta cttccacccc tacatcgcct actgctccaa cgaggtctac caacagcgca 361 cgctgcagaa gctgataagc agcaacgccg ccttccgaga ggccctgaga gagattgaga 421 ggcggccggc gtgcgggggc ctgcccatgc tctccttcct gatcctcccc atgcagcggg 481 tgacccggct gcccctcctg atggatacgc tctgcctcaa gacccagggc cactccgaaa 541 ggtacaaggc tgccagccgt gcactgaagg ccatcagcaa gctggtgagg cagtgcaacg 601 agggggccca caggatggag cgcatggagc agatgtacac gctgcacaca cagctggact 661 tcagcaaggt caagtccctc ccactgatct ccgcctcccg gtggctgctg aagcgcggag 721 agctgttctt agtggaagaa accggacttt ttcgaaaaat tgccagccgg ccaacgtgct 781 accttttcct gttcaacgat gtcctggttg tgaccaagaa gaagagcgag gagagctaca 841 tggtccagga ctacgcccag atgaaccaca tccaggtaga gaagatagag ccgtcTGAGC 901 TCCCTCTGCC CGGGGGCGGC AACCGTAGCT CCTCCGTGCC CCACCCCTTC CAGGTGACCC 961 TGCTTCGCAA CAGCGAGGGC CGCCAGGAGC AGCTCCTGCT CTCCTCGGAC TCCGCGAGTG 1021 ACCGGGCACG GTGGATCGTG GCGCTCACAC ACAGTGAGAG ACAGTGGCAG GGCCTCTCCA 1081 GCAAAGGAGA CCTGCCCCAG GTGGAGATCA CCAAGGCCTT CTTCGCGAAG CAAGCAGACG 1141 AGGTCACACT GCAGCAGGCG GACGTGGTCC TGGTTCTGCA GCAGGAGGAT GGGTGGCTCT 1201 ATGGCGAGAG GCTCCGGGAC GGAGAGACGG GATGGTTCCC CGAGGACTTT GCCCGCTTCA 1261 TCACCAGCCG TGTGGCCGTG GAGGGCAATG TCCGCAGGAT GGAGCGTCTG CGGGTGGAGA 1321 CGGACGTGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAAGGG ACCGCTCACG GTCCCATGAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt