Transcript: Human NM_014448.4

Homo sapiens Rho guanine nucleotide exchange factor 16 (ARHGEF16), mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ARHGEF16 (27237)
Length:
2822
CDS:
167..2296

Additional Resources:

NCBI RefSeq record:
NM_014448.4
NBCI Gene record:
ARHGEF16 (27237)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014448.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000308159 GAGGCACCAATAGCGATTATT pLKO_005 2628 3UTR 100% 15.000 21.000 N ARHGEF16 n/a
2 TRCN0000296257 AGATCCTCACGTCGGAGTTCT pLKO_005 1038 CDS 100% 4.950 6.930 N ARHGEF16 n/a
3 TRCN0000296256 ACCCAACAGCTGATCCCTAAG pLKO_005 419 CDS 100% 6.000 4.200 N ARHGEF16 n/a
4 TRCN0000047507 GCTGGACTTCAGCAAGGTCAA pLKO.1 1618 CDS 100% 4.050 2.835 N ARHGEF16 n/a
5 TRCN0000289517 GCTGGACTTCAGCAAGGTCAA pLKO_005 1618 CDS 100% 4.050 2.835 N ARHGEF16 n/a
6 TRCN0000296255 TGACGACATCCTCGATGAGTC pLKO_005 859 CDS 100% 4.050 2.835 N ARHGEF16 n/a
7 TRCN0000047503 GCAGAAGCTGATAAGCAGCAA pLKO.1 1330 CDS 100% 2.640 1.848 N ARHGEF16 n/a
8 TRCN0000047504 AGCTGTTCTTAGTGGAAGAAA pLKO.1 1686 CDS 100% 0.563 0.394 N ARHGEF16 n/a
9 TRCN0000047505 GCGGGTGGAGACGGACGTGTA pLKO.1 2275 CDS 100% 0.000 0.000 N ARHGEF16 n/a
10 TRCN0000251391 ACTGCTCCAACGAGGTCTATC pLKO_005 1296 CDS 100% 10.800 7.560 N Arhgef16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014448.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11857 pDONR223 100% 59.2% 59.2% None (many diffs) n/a
2 ccsbBroad304_11857 pLX_304 0% 59.2% 59.2% V5 (many diffs) n/a
3 TRCN0000480706 TAAGGGACCGCTCACGGTCCCATG pLX_317 34.6% 59.2% 59.2% V5 (many diffs) n/a
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