Construct: ORF TRCN0000480712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001948.1_s317c1
- Derived from:
- ccsbBroadEn_02955
- DNA Barcode:
- ATCTAAAATCCATCACGACCTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYCBP (26292)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26292 | MYCBP | MYC binding protein | NM_012333.5 | 100% | 100% | |
| 2 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037635.1 | 11.4% | (many diffs) | |
| 3 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037636.1 | 10.5% | (many diffs) | |
| 4 | human | 26292 | MYCBP | MYC binding protein | NR_037632.1 | 9.6% | 1_73del;160_161ins59;324_2524del | |
| 5 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037634.1 | 6.9% | (many diffs) | |
| 6 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037633.1 | 5.8% | (many diffs) | |
| 7 | mouse | 56309 | Mycbp | MYC binding protein | NM_019660.3 | 95.1% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCATTA CAAAGCCGCC GACTCGAAGC 121 GTGAGCAGTT CCGGAGGTAC TTGGAGAAGT CGGGGGTGCT GGACACGCTG ACCAAGGTGT 181 TGGTAGCCTT ATATGAAGAA CCAGAGAAAC CTAACAGTGC TTTGGATTTT TTAAAGCATC 241 ACTTAGGAGC TGCTACTCCA GAAAATCCAG AAATAGAGCT GCTTCGCCTA GAACTGGCCG 301 AAATGAAAGA GAAGTATGAA GCTATTGTAG AAGAAAATAA AAAACTGAAA GCAAAGCTTG 361 CTCAGTATGA ACCACCTCAG GAGGAGAAGC GTGCTGAATA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAATCTAA AATCCATCAC GACCTCTTAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt