Construct: ORF TRCN0000480720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015711.2_s317c1
- Derived from:
- ccsbBroadEn_06732
- DNA Barcode:
- ATTCTGAGATCGGGAAGACTGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKNOX1 (5316)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480720
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5316 | PKNOX1 | PBX/knotted 1 homeobox 1 | NM_004571.5 | 99.9% | 100% | 342T>C |
| 2 | human | 5316 | PKNOX1 | PBX/knotted 1 homeobox 1 | NM_001320694.2 | 99.6% | 99.7% | 342T>C;519_520insCAG |
| 3 | human | 5316 | PKNOX1 | PBX/knotted 1 homeobox 1 | NM_001286258.1 | 73.1% | 73.1% | 0_1ins351 |
| 4 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | NM_016670.3 | 87.5% | 96.1% | (many diffs) |
| 5 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | XM_006523854.2 | 87.5% | 96.1% | (many diffs) |
| 6 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | XM_006523855.2 | 87.5% | 96.1% | (many diffs) |
| 7 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | XM_006523856.3 | 87.3% | 95.8% | (many diffs) |
| 8 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | XM_006523857.3 | 87.3% | 95.8% | (many diffs) |
| 9 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | XM_006523858.1 | 87.1% | 95.6% | (many diffs) |
| 10 | mouse | 18771 | Pknox1 | Pbx/knotted 1 homeobox | NR_027493.1 | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggctacacag acattaagta tagacagcta tcaagatggg caacagatgc 121 aagtagtaac agagttaaag acagaacaag atccaaactg ctctgaaccc gatgcagaag 181 gagtgagccc tccccctgtg gagtctcaga ccccgatgga tgtggacaag caggccattt 241 ataggcatcc actatttcca ttattagctt tgttgtttga aaaatgtgaa caatctacac 301 agggctctga aggcacaact tctgccagtt ttgatgtaga catcgaaaat tttgtaagaa 361 agcaagagaa ggaagggaaa cctttctttt gtgaagatcc agaaaccgat aatttaatgg 421 taaaagcaat ccaggttttg cgcattcatc ttcttgagct ggaaaaggtt aacgaactct 481 gcaaagattt ctgcagtcga tacattgctt gtctgaaaac aaaaatgaac agtgaaactc 541 tgttgagtgg agagcctgga agcccgtact caccagtgca gtcccagcag attcaaagtg 601 ccatcacagg caccatcagc cctcagggaa ttgtggtgcc ggcgtccgcg ctgcagcagg 661 gaaacgtagc catggcgacg gtggcaggtg gcacagtgta tcagcctgtc acggtcgtca 721 ctccccaagg ccaagtggtc acacagacat tgtcgcctgg gacaattagg atccagaact 781 cccagcttca gttacagtta aaccaagatc tcagcatctt gcatcaagat gatggttcat 841 ctaagaacaa gaggggcgtc ctgccaaagc atgccacgaa cgtgatgcgg tcctggctct 901 tccagcacat cgggcatccc tacccaacag aggatgagaa aaaacagatt gctgctcaga 961 caaatttgac actacTCCAA GTCAACAACT GGTTCATCAA TGCCAGAAGA CGAATTCTTC 1021 AGCCAATGTT GGATTCAAGT TGTTCAGAGA CCCCCAAAAC AAAGAAAAAA ACTGCTCAGA 1081 ACCGGCCAGT TCAGAGGTTT TGGCCTGATT CTATTGCATC AGGAGTCGCA CAGCCACCGC 1141 CGAGCGAGCT CACCATGTCG GAAGGAGCTG TTGTCACCAT CACCACGCCC GTGAACATGA 1201 ACGTGGACAG CCTTCAGTCT CTGTCCTCGG ACGGGGCCAC CCTGGCGGTG CAGCAGGTCA 1261 TGATGGCAGG GCAGAGCGAG GACGAGTCTG TGGACAGCAC AGAGGAGGAT GCGGGTGCCC 1321 TGGCCCCTGC CCACATCAGC GGGCTGGTCT TGGAGAACAG TGACTCCCTG CAGTACCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAA TTCTGAGATC GGGAAGACTG ACAACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att