Construct: ORF TRCN0000480735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004758.2_s317c1
- Derived from:
- ccsbBroadEn_07731
- DNA Barcode:
- GGCTAGCTGGATTGCCGGCAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAP1 (11033)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11033 | ADAP1 | ArfGAP with dual PH domains 1 | NM_006869.4 | 99.8% | 99.4% | 10G>A;721G>A |
| 2 | human | 11033 | ADAP1 | ArfGAP with dual PH domains 1 | NM_001284308.1 | 92% | 90% | (many diffs) |
| 3 | human | 11033 | ADAP1 | ArfGAP with dual PH domains 1 | NM_001284309.1 | 80.7% | 80.7% | 0_1ins216 |
| 4 | human | 11033 | ADAP1 | ArfGAP with dual PH domains 1 | NM_001284310.1 | 80.7% | 80.7% | 0_1ins216 |
| 5 | human | 11033 | ADAP1 | ArfGAP with dual PH domains 1 | NM_001284311.1 | 74.1% | 72.7% | (many diffs) |
| 6 | mouse | 231821 | Adap1 | ArfGAP with dual PH domains 1 | NM_172723.4 | 88.3% | 93.8% | (many diffs) |
| 7 | mouse | 231821 | Adap1 | ArfGAP with dual PH domains 1 | XM_006504669.2 | 71.5% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagaagcgg cgcagggcgg tcctggagct gctgcagcgg ccggggaacg 121 cgcgctgcgc ggactgcggc gccccggatc ccgactgggc ctcctacact ctgggcgtct 181 tcatctgcct gagctgctcg ggaatccacc ggaatatccc ccaggtcagc aaggtgaagt 241 ccgtccgcct ggacgcctgg gaggaggccc aagtggagtt catggcctcc cacgggaacg 301 acgccgcgag agccaggttt gagtccaaag taccctcctt ctactaccgg cccacgccct 361 ccgactgcca gctccttcga gagcagtgga tccgggccaa gtacgagcga caggagttca 421 tctacccgga gaagcaggag ccctactcgg cagggtaccg tgagggtttt ctctggaagc 481 gtggccggga caacgggcag tttttgagcc ggaagtttgt gctgacagaa cgagagggtg 541 ctctgaagta tttcaacaga aatgatgcca aggagcccaa ggccgtgatg aagatcgagc 601 acctgaacgc caccttccag ccggccaaga tcggccaccc ccacggcctg caggtcacct 661 acctgaagga caacagcacc cgtaacatct tcatctacca tgaggacggg aaggagattg 721 tggactggtt caatgcacTC CGAGCTGCTC GCTTCCACTA CCTGCAGGTG GCATTCCCAG 781 GGGCCAGCGA CGCAGATCTG GTGCCAAAGC TCTCCAGGAA CTACCTGAAG GAAGGCTACA 841 TGGAGAAGAC GGGGCCCAAG CAAACGGAAG GCTTCCGGAA GCGCTGGTTC ACCATGGATG 901 ACCGCAGGCT CATGTACTTC AAAGACCCCC TGGACGCCTT CGCCCGAGGG GAAGTCTTCA 961 TTGGCAGCAA GGAGAGTGGC TACACGGTGC TGCATGGGTT CCCGCCGTCC ACCCAGGGCC 1021 ACCACTGGCC ACATGGCATC ACCATCGTCA CGCCCGACCG CAAGTTTCTG TTTGCCTGCG 1081 AGACGGAGTC CGACCAGAGG GAGTGGGTGG CGGCCTTCCA GAAGGCGGTG GACAGGCCCA 1141 TGCTGCCCCA GGAGTACGCA GTGGAGGCGC ACTTCAAGCA TAAACCTGAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAGGCTAGC TGGATTGCCG GCAGTAAACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt