Construct: ORF TRCN0000480761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012652.2_s317c1
- Derived from:
- ccsbBroadEn_01166
- DNA Barcode:
- CTTTTTCCCATACTAGCCCAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDK2 (5164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_002611.5 | 100% | 100% | |
| 2 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199898.1 | 84.2% | 84.2% | 0_1ins192 |
| 3 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199899.1 | 84.2% | 84.2% | 0_1ins192 |
| 4 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199900.1 | 46% | 40.5% | (many diffs) |
| 5 | mouse | 18604 | Pdk2 | pyruvate dehydrogenase kina... | NM_133667.2 | 91.5% | 97.5% | (many diffs) |
| 6 | mouse | 18604 | Pdk2 | pyruvate dehydrogenase kina... | XM_006532457.3 | 77% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1287
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ctgggtgtgg gcgctgctga agaatgcgtc cctggcaggg gcgcccaagt 121 acatagagca cttcagcaag ttctccccgt ccccgctgtc catgaagcag tttctggact 181 tcggatccag caatgcctgt gagaaaacct ccttcacctt cctcaggcag gagctgcctg 241 tgcgcctggc caacatcatg aaagagatca acctgcttcc cgaccgagtg ctgagcacac 301 cctccgtgca gctggtgcag agctggtatg tccagagcct cctggacatc atggagttcc 361 tggacaagga tcccgaggac catcgcaccc tgagccagtt cactgacgcc ctggtcacca 421 tccggaaccg gcacaacgac gtggtgccca ccatggcaca aggcgtgctt gagtacaagg 481 acacctacgg cgatgacccc gtctccaacc agaacatcca gtacttcctg gaccgcttct 541 acctcagccg catctccatc cgcatgctca tcaaccagca caccctcatc tttgatggca 601 gcaccaaccc agcccatccc aaacacatcg gcagcatcga ccccaactgc aacgtctctg 661 aggtggtcaa agatgcctac gacatggcta agctcctgtg tgacaagtat tacatggcct 721 cacctgacct ggagatccag gagatcaatg cagccaactc caaacagccg attcacatgg 781 tctacgtccc ctcccacctc taccacatgc tctttgagct cttcaagaat gccatgaggg 841 cgactgtgga aagccatgag tccagccTCA TTCTCCCACC CATCAAGGTC ATGGTGGCCT 901 TGGGTGAGGA AGATCTGTCC ATCAAGATGA GTGACCGAGG TGGGGGTGTT CCCTTGAGGA 961 AGATTGAGCG ACTCTTCAGC TACATGTACT CCACAGCACC CACCCCCCAG CCTGGCACCG 1021 GGGGAACGCC GCTGGCTGGC TTTGGTTATG GGCTCCCCAT TTCCCGCCTC TACGCCAAGT 1081 ACTTCCAGGG AGACCTGCAG CTCTTCTCCA TGGAAGGCTT TGGGACCGAT GCTGTCATCT 1141 ATCTCAAGGC CCTGTCCACG GACTCGGTGG AGCGCCTGCC TGTCTACAAC AAGTCAGCCT 1201 GGCGCCACTA CCAGACCATC CAGGAGGCCG GCGACTGGTG TGTGCCCAGC ACGGAGCCCA 1261 AGAACACGTC CACGTACCGC GTCAGCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTTTTTCC 1441 CATACTAGCC CAATTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt