Construct: ORF TRCN0000480785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001712.1_s317c1
- Derived from:
- ccsbBroadEn_11609
- DNA Barcode:
- TAGCCACTCCTTATCTACCACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR6 (11180)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11180 | WDR6 | WD repeat domain 6 | NM_001320547.1 | 27% | 27% | 1_2343del |
| 2 | human | 11180 | WDR6 | WD repeat domain 6 | NM_001320546.2 | 26.3% | 26.3% | 1_2418del |
| 3 | human | 11180 | WDR6 | WD repeat domain 6 | NM_018031.6 | 25.7% | 25.7% | 1_2496del |
| 4 | mouse | 83669 | Wdr6 | WD repeat domain 6 | NM_031392.2 | 22.1% | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 933
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cctttcgtcc caccggctag atgagtattg ggaccggcaa cgcaatcggc 121 atcggatggt taaggtagac ccagagacca ggtacatgtc ccttgctgtg tgtgaacttg 181 accagcccgg ccttggcccc cttgtggctg cagcctgtag tgatggggcc gtaaggctct 241 ttcttttgca ggattctggg cggattctgc agctccttgc tgaaaccttc caccataagc 301 gatgtgtcct caaggtccac tcctttacac acgaggcacc caaccagagg cggaggctcc 361 tcctgtgcag cgcagctact gatggcagcc tggctttctg ggatctcacc accatgctag 421 accatgactc cactgtcctg gagcctccag tggatcctgg gcttccctac cggcttggca 481 ccccctccct gactctccag gcccacagct gtggtatcaa cagcctgcac accttgccca 541 cccgtgaggg ccaccatctc gtggccagtg gcagtgaaga tggatccctc catgtcttcg 601 tgcttgctGT GGAGATGCTA CAGCTAGAAG AGGCTGTGGG AGAGGCTGGG CTGGTACCCC 661 AGCTGCGTGT GCTAGAGGAA TACTCTGTCC CCTGTGCACA TGCTGCCCAT GTGACAGGCC 721 TCAAGATCCT AAGCCCAAGC ATCATGGTCT CAGCCTCCAT TGATCAACGG CTGACCTTCT 781 GGCGTCTGGG GCATGGTGAA CCCACCTTCA TGAATAGCAC TGTGTTCCAT GTGCCTGATG 841 TGGCTGACAT GGACTGCTGG CCTGTGAGCC CTGAGTTTGG CCACCGTTGT GCCCTTGGGG 901 GTCAGGGGCT TGAGGTTTAC AACTGGTATG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 961 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1021 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1081 GCCACTCCTT ATCTACCACA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1141 ttgtgaaaga tt