Construct: ORF TRCN0000480786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001690.1_s317c1
- Derived from:
- ccsbBroadEn_03555
- DNA Barcode:
- CGGTTTGGCCTGTAAAGGACGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PANK4 (55229)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55229 | PANK4 | pantothenate kinase 4 (inac... | NM_018216.4 | 99.9% | 100% | 393A>G |
| 2 | human | 55229 | PANK4 | pantothenate kinase 4 (inac... | XR_241034.3 | 85.6% | (many diffs) | |
| 3 | human | 55229 | PANK4 | pantothenate kinase 4 (inac... | XM_011541708.3 | 52.7% | 52.6% | (many diffs) |
| 4 | mouse | 269614 | Pank4 | pantothenate kinase 4 | NM_172990.5 | 85.4% | 92.2% | (many diffs) |
| 5 | mouse | 269614 | Pank4 | pantothenate kinase 4 | NM_001305804.1 | 80.6% | 86.9% | (many diffs) |
| 6 | mouse | 269614 | Pank4 | pantothenate kinase 4 | NR_131230.1 | 74.7% | (many diffs) | |
| 7 | mouse | 269614 | Pank4 | pantothenate kinase 4 | XM_017320246.1 | 42.1% | 44.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2385
- ORF length:
- 2319
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtgtgga gcgagcggca gcgggagcag cggggacagt ctggacaaga 121 gcatcacgct gccccccgac gagatcttcc gcaacctgga gaacgccaag cgcttcgcca 181 tcgacatagg cgggtcgtta accaagctgg cctactattc aacggtacag cacaaagtcg 241 ccaaggtgcg gtctttcgac cactccggaa aggacacaga acgtgaacat gagccgccct 301 atgagatttc agttcaagaa gagatcactg ctcgactgca cttcattaag tttgagaata 361 cctacatcga agcctgcctg gacttcatca aagaccatct cgtcaacaca gagaccaagg 421 tcatccaggc gaccgggggc ggggcctaca agttcaagga cctcatcgaa gagaagctgc 481 ggctgaaagt cgacaaggag gacgtgatga cgtgcctgat taaggggtgc aacttcgtgc 541 tcaagaacat cccccatgag gccttcgtgt accagaagga ttccgaccct gagttccggt 601 tccagaccaa ccacccccac attttcccct atcttcttgt caatatcggc tctggagtct 661 ccatcgtgaa ggtggagacg gaggacaggt tcgagtgggt cggcggcagc tccattggag 721 gcggcacctt ctgggggctt ggcgctctgc tcaccaaaac gaagaagttt gacgagctcc 781 tgcacctggc ctcgaggggc cagcacagca atgtggacat gctggtgcgg gacgtctacg 841 gcggcgccca ccagactctc gggctgagcg ggaacctcat cgccagcagc ttcgggaagt 901 cggccaccgc cgaccaagag ttctccaaag aagacatggc gaagagcctg ctgcacatga 961 tcagcaacga cattgggcag ctggcctgcc tccacgcacg gctgcacagc ctggaccgcg 1021 tgtactttgg aggcttcttt atccggggcc accccgtgac catgcgcacc atcacctata 1081 gcatcaactt cttctccaag ggggaagtgc aggcgctgtt tctgaggcac gaaggctacc 1141 tgggagccat cggagcgttc ctgaaaggag ctgagcagga caatcctaac cagtacagct 1201 ggggagagaa ctatgcaggc agctccgggc tgatgagtgc atcacccgag ctcggcccgg 1261 cgcagcgggc gcggagtggc acttttgact tgctggaaat ggaccggctg gagaggccac 1321 tggttgacct gccgctcctc ctggacccgc cctcctacgt gcccgacacg gtggacctca 1381 ccgatgacgc tctggcccga aaatactggc tcacctgctt tgaggaggcc ctggacgggg 1441 tagtgaagcg cgcagtggcg agccagccag actctgtgga tgcagccgag agggcggaga 1501 agttccggca gaagtactgg aacaagcttc agaccctgag gcagcagccc ttcgcctatg 1561 ggaccctgac cgtgcgcagc ctgctggaca ccagggagca ctgtctgaac gagttcaact 1621 tcccggatcc ctactccaaa gtgaagcagc gggagaatgg cgtggcgctg aggtgcttcc 1681 ccggggtcgt gcgctccctg gacgcgctgg gctgggagga acggcagctg gcgctggtga 1741 aaggcctcct ggcggggaat gtcttcgact ggggggccaa agccgtgtct gctgtccttg 1801 aatccgaccc ctactttggg tttgaagaag caaagaggaa gttacaagaa agaccctggc 1861 tcgtggattc ctacagcgag tggcttcaga gattaaaggg gccccctcat aaatgtgcct 1921 taattttcgc agataacagt ggaatagaca tcattttggg agtcttcccc TTTGTCAGGG 1981 AGCTACTCCT TAGAGGGACA GAGGTCATCC TGGCGTGCAA CTCAGGCCCC GCCCTGAACG 2041 ACGTGACCCA CAGCGAGTCC CTCATCGTGG CAGAGCGTAT TGCGGGCATG GACCCTGTCG 2101 TGCACTCTGC GCTCCAGGAA GAGAGGCTGC TGCTGGTGCA GACGGGCTCC AGCTCCCCGT 2161 GCCTCGACCT CAGCCGCCTG GATAAGGGGC TGGCCGCACT GGTGCGGGAG CGTGGCGCGG 2221 ATCTGGTGGT CATCGAGGGC ATGGGCCGTG CTGTCCACAC AAACTACCAC GCAGCCCTGC 2281 GCTGCGAGAG CCTCAAGCTG GCCGTCATCA AGAACGCGTG GCTGGCCGAG CGGCTGGGCG 2341 GCCGGCTCTT CAGCGTCATC TTCAAGTACG AGGTCCCAGC CGAGTGCCCA ACTTTCTTGT 2401 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2461 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2521 GAAAGGACGA CGGTTTGGCC TGTAAAGGAC GACCACGCGT TAAGTCgaca atcaacctct 2581 ggattacaaa atttgtgaaa gatt