Construct: ORF TRCN0000480806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010734.1_s317c1
- Derived from:
- ccsbBroadEn_04476
- DNA Barcode:
- GGTATCATGTAGTCGGCATGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIL4 (85313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85313 | PPIL4 | peptidylprolyl isomerase li... | NM_139126.4 | 100% | 100% | |
| 2 | mouse | 67418 | Ppil4 | peptidylprolyl isomerase (c... | NM_026141.3 | 91% | 96.7% | (many diffs) |
| 3 | mouse | 67418 | Ppil4 | peptidylprolyl isomerase (c... | XR_001779581.1 | 64.9% | (many diffs) | |
| 4 | mouse | 67418 | Ppil4 | peptidylprolyl isomerase (c... | XM_006512832.3 | 62.2% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1542
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggttctactg gagaccactt taggcgacgt cgtcatcgac ttgtacaccg 121 aagaacggcc gcgtgcctgc ttgaatttct tgaaactgtg caaaataaaa tattacaatt 181 attgccttat tcacaatgta cagagggatt ttatcataca aactggcgat cctacaggga 241 ctggccgtgg aggagagtct atctttggcc aactgtatgg tgatcaagca agcttttttg 301 aggcagaaaa agtcccaaga attaagcaca agaagaaagg cacagtgtcc atggtgaata 361 atggcagtga tcaacatgga tctcagtttc ttatcaccac aggagaaaat ctagattatc 421 ttgatggtgt ccatacggtg tttggtgagg tgacagaagg catggacata attaagaaaa 481 ttaatgagac ctttgttgac aaggactttg taccatatca ggatatcagg ataaatcata 541 cggtgatttt agatgatcca tttgatgacc ctcctgattt attaatccct gatcgatcac 601 cagaacctac aagggaacaa ttagatagtg gtcgaatagg agcagatgaa gaaattgatg 661 atttcaaagg aagatcagct gaggaagtag aagaaataaa ggcagaaaaa gaggctaaaa 721 ctcaggctat acttttggag atggtgggag acctacctga tgcagatatt aaacctccag 781 aaaatgtact gtttgtgtgt aaattgaacc cagtgaccac agatgaggat ctggaaataa 841 tattctctag atttgggcca ataagaagtt gtgaagttat ccgagactgg aagacaggag 901 agtccctctg ttacgctttt attgaatttg aaaaggaaga agattgtgag aaagcattct 961 tcaaaatgga caatgtgctt atagatgaca gaagaataca tgtggatttt agccagtcgg 1021 ttgcaaaggt taaatggaaa ggaaaaggtg ggaaatacac caagagtgat ttcaaggagt 1081 atgaaaaaga acaggataaa ccacctaatt tggttcTGAA AGATAAAGTA AAGCCCAAAC 1141 AGGATACAAA ATACGATCTT ATATTAGATG AGCAGGCCGA AGACTCAAAA TCAAGTCACT 1201 CACACACAAG TAAAAAACAC AAGAAGAAAA CCCATCACTG TTCTGAAGAG AAAGAAGATG 1261 AGGACTACAT GCCAATCAAA AATACTAATC AGGATATCTA TAGAGAAATG GGGTTTGGTC 1321 ACTATGAAGA AGAAGAAAGC TGTTGGGAGA AACAAAAGAG TGAAAAGAGA GACCGAACTC 1381 AGAACCGAAG TCGTAGCCGA TCTCGAGAGA GGGATGGCCA TTATAGTAAT AGTCATAAAT 1441 CAAAATACCA AACAGATCTT TATGAAAGAG AAAGGAGTAA AAAGAGAGAC CGAAGCAGAA 1501 GTCCAAAGAA GTCCAAAGAT AAAGAAAAAT CTAAGTATAG ATGCCCAACT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGAGGT ATCATGTAGT CGGCATGCCT TACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t