Construct: ORF TRCN0000480812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009763.2_s317c1
- Derived from:
- ccsbBroadEn_06921
- DNA Barcode:
- GCTTCGGCTGTTAAGTCCGCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL23 (6368)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480812
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | NM_145898.4 | 100% | 100% | |
| 2 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | NM_005064.6 | 87.5% | 86.8% | 137_187del |
| 3 | human | 6359 | CCL15 | C-C motif chemokine ligand 15 | NM_032965.6 | 81.8% | 68.3% | (many diffs) |
| 4 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | XR_429910.3 | 37.9% | (many diffs) | |
| 5 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027922.3 | 21% | (many diffs) | |
| 6 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027921.3 | 20.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 429
- ORF length:
- 360
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GAAGGTCTCC GTGGCTGCCC TCTCCTGCCT CATGCTTGTT ACTGCCCTTG 121 GATCCCAGGC CCGGGTCACA AAAGATGCAG AGACAGAGTT CATGATGTCA AAGCTTCCAT 181 TGGAAAATCC AGTACTTCTG GACAGATTCC ATGCTACTAG TGCTGACTGC TGCATCTCCT 241 ACACCCCACG AAGCATCCCG TGTTCACTCC TGGAGAGTTA CTTTGAAACG AACAGCGAGT 301 GCTCCAAGCC GGGTGTCATC TTCCTCACCA AGAAGGGGCG ACGTTTCTGT GCCAACCCCA 361 GTGATAAGCA AGTTCAGGTT TGCATGAGAA TGCTGAAGCT GGACACACGG ATCAAGACCA 421 GGAAGAATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTTCG GCTGTTAAGT CCGCCTTCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt