Construct: ORF TRCN0000480818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006115.2_s317c1
- Derived from:
- ccsbBroadEn_05932
- DNA Barcode:
- TAACAAGCTTTACGTTTGTATAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CAPG (822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 822 | CAPG | capping actin protein, gels... | NM_001256139.1 | 99.8% | 99.7% | 813A>C;1004A>G |
| 2 | human | 822 | CAPG | capping actin protein, gels... | NM_001320732.1 | 99.8% | 99.7% | 813A>C;1004A>G |
| 3 | human | 822 | CAPG | capping actin protein, gels... | NM_001320733.1 | 99.8% | 99.7% | 813A>C;1004A>G |
| 4 | human | 822 | CAPG | capping actin protein, gels... | NM_001747.4 | 99.8% | 99.7% | 813A>C;1004A>G |
| 5 | human | 822 | CAPG | capping actin protein, gels... | XM_011533122.1 | 99.8% | 99.7% | 813A>C;1004A>G |
| 6 | human | 822 | CAPG | capping actin protein, gels... | XM_011533123.1 | 99.8% | 99.7% | 813A>C;1004A>G |
| 7 | human | 822 | CAPG | capping actin protein, gels... | NM_001256140.1 | 95.4% | 95.4% | 617_618ins45;768A>C;959A>G |
| 8 | human | 822 | CAPG | capping actin protein, gels... | NM_001320734.1 | 90.9% | 90.8% | 664_665ins93;720A>C;911A>G |
| 9 | mouse | 12332 | Capg | capping protein (actin fila... | NM_001042534.3 | 89.2% | 92.8% | (many diffs) |
| 10 | mouse | 12332 | Capg | capping protein (actin fila... | NM_001271395.1 | 89.2% | 92.8% | (many diffs) |
| 11 | mouse | 12332 | Capg | capping protein (actin fila... | NM_001271415.1 | 89.2% | 92.8% | (many diffs) |
| 12 | mouse | 12332 | Capg | capping protein (actin fila... | NM_007599.4 | 89.2% | 92.8% | (many diffs) |
| 13 | mouse | 12332 | Capg | capping protein (actin fila... | XM_017321370.1 | 89.2% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 acagcatgta cacagccatt ccccagagtg gctctccatt cccaggctca gtgcaggatc 121 caggcctgca tgtgtggcgg gtggagaagc tgaagccggt gcctgtggcg caagagaacc 181 agggcgtctt cttctcgggg gactcctacc tagtgctgca caatggccca gaagaggttt 241 cccatctgca cctgtggata ggccagcagt catcccggga tgagcagggg gcctgtgccg 301 tgctggctgt gcacctcaac acgctgctgg gagagcggcc tgtgcagcac cgcgaggtgc 361 agggcaatga gtctgacctc ttcatgagct acttcccacg gggcctcaag taccaggaag 421 gtggtgtgga gtcagcattt cacaagacct ccacaggagc cccagctgcc atcaagaaac 481 tctaccaggt gaaggggaag aagaacatcc gtgccaccga gcgggcactg aactgggaca 541 gcttcaacac tggggactgc ttcatcctgg acctgggcca gaacatcttc gcctggtgtg 601 gtggaaagtc caacatcctg gaacgcaaca aggcgaggga cctggccctg gccatccggg 661 acagtgagcg acagggcaag gcccaggtgg agattgtcac tgatggggag gagcctgctg 721 agatgatcca ggtcctgggc cccaagcctg ctctgaagga gggcaaccct gaggaagacc 781 tcacagctga caaggcaaat gcccaggccg cagctctgta taaggtctct gatgccactg 841 gacagatgaa cctgaccaag gtggctgact ccagcccctt tgcccttgaa ctgctgatat 901 cTGATGACTG CTTTGTGCTG GACAACGGGC TCTGTGGCAA GATCTATATC TGGAAGGGGC 961 GAAAAGCGAA TGAGAAGGAG CGGCAGGCAG CCCTGCAGGT GGCCGAGGGC TTCATCTCGC 1021 GCATGCAGTA CGCCCCGAAC ACTCAGGTGG AGATTCTGCC TCAGGGCCGT GAGAGTCCCA 1081 TCTTCAAGCA ATTTTTCAAG GACTGGAAAT GAGGGTGGGC GTCTTCCTGC CCCATGCTCC 1141 CCTGCCCCCC ACCACCTGCC TGCTTGCTTC TCTGGCTGCC TGGTCAGTGC AGAGGTGCCC 1201 CCTGCAGATG TTCAATAAAG GAGACAAGTG CTTTAAAAAA AAAAAAAAAA AACTCGAGGC 1261 ATCTATGTCG GGTGCGGAGA AAGAGGTAAT GAAATGGCAC ATGGTCATAG CTGTTTCCTG 1321 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGATAACA AGCTTTACGT TTGTATAGTA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt