Transcript: Mouse NM_001271395.1

Mus musculus capping protein (actin filament), gelsolin-like (Capg), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Capg (12332)
Length:
1393
CDS:
236..1285

Additional Resources:

NCBI RefSeq record:
NM_001271395.1
NBCI Gene record:
Capg (12332)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001271395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000091422 TGGACAGATGAATCTGACCAA pLKO.1 1012 CDS 100% 2.640 3.696 N Capg n/a
2 TRCN0000318309 TGGACAGATGAATCTGACCAA pLKO_005 1012 CDS 100% 2.640 3.696 N Capg n/a
3 TRCN0000091419 GAGTCCCATCTTCAAGCAATT pLKO.1 1246 CDS 100% 10.800 7.560 N Capg n/a
4 TRCN0000091420 TCCCATCTTCAAGCAATTCTT pLKO.1 1249 CDS 100% 5.625 3.938 N Capg n/a
5 TRCN0000318310 TCCCATCTTCAAGCAATTCTT pLKO_005 1249 CDS 100% 5.625 3.938 N Capg n/a
6 TRCN0000091418 TCCTACCTAGTGCTTCACAAT pLKO.1 374 CDS 100% 4.950 3.465 N Capg n/a
7 TRCN0000318307 TCCTACCTAGTGCTTCACAAT pLKO_005 374 CDS 100% 4.950 3.465 N Capg n/a
8 TRCN0000091421 CCAGCCGAGATGATTCAGGTT pLKO.1 887 CDS 100% 2.640 1.848 N Capg n/a
9 TRCN0000318308 CCAGCCGAGATGATTCAGGTT pLKO_005 887 CDS 100% 2.640 1.848 N Capg n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05932 pDONR223 99.3% 89.2% 92.8% None (many diffs) n/a
2 ccsbBroad304_05932 pLX_304 0% 89.2% 92.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000480818 TAACAAGCTTTACGTTTGTATAGT pLX_317 33.6% 89.2% 92.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000470895 CTAATGCACCCCTCACCGTGAAGT pLX_317 33.6% 89.2% 92.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_05931 pDONR223 100% 89.2% 92.8% None (many diffs) n/a
6 ccsbBroad304_05931 pLX_304 0% 89.2% 92.8% V5 (many diffs) n/a
7 TRCN0000466938 CTGACTCAGCCGCGCGCCGTCCTA pLX_317 43.4% 89.2% 92.8% V5 (many diffs) n/a
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