Construct: ORF TRCN0000480850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008702.1_s317c1
- Derived from:
- ccsbBroadEn_03180
- DNA Barcode:
- TTGCAGGATTCGATTTTCGGGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DESI2 (51029)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | NM_016076.5 | 100% | 100% | |
| 2 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | XM_011544205.3 | 94.8% | 94.3% | (many diffs) |
| 3 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | XM_011544203.3 | 89.8% | 87.6% | (many diffs) |
| 4 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | NM_001297746.2 | 82.9% | 82.9% | 113_114ins99 |
| 5 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | XM_011544207.2 | 77.9% | 77.3% | (many diffs) |
| 6 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | XM_011544206.3 | 74.2% | 72% | (many diffs) |
| 7 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | XR_001737208.2 | 49.4% | (many diffs) | |
| 8 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | NR_123735.2 | 14.3% | 1_188del;397_398insAGAAG;766_4012del | |
| 9 | human | 51029 | DESI2 | desumoylating isopeptidase 2 | NR_123736.2 | 12.1% | 1_188del;303_304ins94;677_3923del | |
| 10 | mouse | 78825 | Desi2 | desumoylating isopeptidase 2 | NM_024282.3 | 90.7% | 96.9% | (many diffs) |
| 11 | mouse | 78825 | Desi2 | desumoylating isopeptidase 2 | XM_006497038.3 | 82.8% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggctaaccag ttagtggtgc tcaacgtgta cgacatgtat tggatgaacg 121 aatatacctc atccattgga attggagttt ttcattcagg aattgaagtc tatggcagag 181 aatttgctta tggtggccat ccttacccct tttctggaat atttgaaatt tccccaggaa 241 atgcttctga actaggagaa aCATTTAAAT TTAAAGAAGC TGTTGTTTTA GGGAGCACGG 301 ACTTCCTAGA AGATGATATA GAAAAAATTG TAGAAGAACT GGGAAAAGAA TACAAAGGCA 361 ATGCTTATCA TTTAATGCAT AAAAACTGCA ATCATTTTTC TTCAGCTTTA TCAGAGATTC 421 TTTGTGGGAA AGAGATTCCT CGCTGGATCA ATCGACTTGC CTACTTCAGC TCCTGTATAC 481 CCTTTCTACA GAGTTGCCTC CCGAAGGAGT GGCTCACGCC CGCAGCCCTG CAGTCTAGTG 541 TCAGCCAAGA ACTCCAGGAT GAACTGGAGG AAGCAGAGGA TGCTGCCGCA TCCGCTTCCG 601 TGGCAAGCAC TGCAGCAGGC TCCAGACCCG GGCGCCACAC TAAACTATAC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGATTGCAGG ATTCGATTTT CGGGCAAACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt