Construct: ORF TRCN0000480853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008446.1_s317c1
- Derived from:
- ccsbBroadEn_09851
- DNA Barcode:
- TTAGTAACGGGGGTCCGAAAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPCN2 (219931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219931 | TPCN2 | two pore segment channel 2 | NM_139075.4 | 99.7% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2325
- ORF length:
- 2256
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaaccc caggcggagt cggagcccct gctgggcggg gcccgcggcg 121 gtggcggcga ctggccggcg gggctgacca cttaccgcag catccaagtc ggccctggtg 181 ccgcggccag gtgggacctc tgcattgatc aggctgtggt cttcatcgaa gatgctattc 241 agtaccgctc catcaaccac cgggtggatg ccagctcgat gtggctttac cgacggtatt 301 actcgaacgt atgccaacgg actttgagct tcaccatctt cttgatcctg tttttggctt 361 ttatcgagac cccatcctca ctcaccagca cggcggacgt gcgctaccgc gctgccccct 421 gggagccgcc ctgcggcctg accgagagtg tcgaggtgct ctgcctgctg gtctttgcgg 481 ccgacctctc tgtgaagggt tacctgttcg ggtgggccca tttccagaaa aacctttggc 541 tgctgggcta cctcgtggtg ctggtggtgt ctctggtgga ctggaccgtg tccctgagtc 601 tcgtgtgtca tgagcccctg cggatccgcc ggcttctccg tcccttcttc ctgctgcaga 661 actcctctat gatgaagaag accttgaaat gcatccgctg gtcgctgccg gaaatggcca 721 gcgtcgggct gctgctggcc atccacctgt gcctcttcac catgttcgga atgctgctgt 781 tcgctggtgg gaagcaggat gatgggcagg acagggagag gctgacctac ttccagaacc 841 tgcctgagtc tctgacttcc ctcctggtgc tgctgaccac ggccaacaac cccgatgtga 901 tgattcctgc gtattccaag aaccgggcct atgccatctt cttcatagtc ttcactgtga 961 taggaagcct gtttctgatg aacctgctga cagccatcat ctacagtcag ttccggggct 1021 acctgatgaa atctctccag acctcgctgt ttcggaggcg gctgggaacc cgggctgcct 1081 ttgaagtcct atcctccatg gtgggggagg gaggagcctt ccctcaggca gttggggtga 1141 agccccagaa cttgctgcag gtgcttcaga aggtccagct ggacagctcc cacaaacagg 1201 ccatgatgga gaaggtgcgt tcctacggca gtgttctgct gtcagctgag gagtttcaga 1261 agctcttcaa cgagcttgac agaagtgtgg ttaaagagca cccgccgagg cccgagtacc 1321 agtctccgtt tctgcagagc gcccagttcc tcttcggcca ctactacttt gactacctgg 1381 ggaacctcat cgccctggca aacctggtgt ccatttgcgt gttcctggtg ctggatgcag 1441 atgtgctgcc tgctgagcgt gatgacttca tcctggggat tctcaactgc gtcttcattg 1501 tgtactacct gttggagatg ctgctcaagg tctttgccct gggcctgcga gggtacctgt 1561 cctaccccag caacgtgttt gacgggctcc tcaccgttgt cctgctggtt ttggagatct 1621 caactctggc tgtgtaccga ttgccacacc caggctggag gccggagatg gtgggcctgc 1681 tgtcgctgtg ggacatgacc cgcatgctga acatgctcat cgtgttccgc ttcctgcgta 1741 tcatccccag catgaagccg atggccgtgg tggccagtac cgtcctgggc ctggtgcaga 1801 acatgcgtgc ttttggcggg atcctggtgg tggtctacta cgtatttgcc atcattggga 1861 tcaacttgtt tagaggcgtc attgtggctc ttcctggaaa cagcagccTG GCCCCTGCCA 1921 ATGGCTCGGC GCCCTGTGGG AGCTTCGAGC AGCTGGAGTA CTGGGCCAAC AACTTCGATG 1981 ACTTTGCGGC TGCCCTGGTC ACTCTGTGGA ACTTGATGGT GGTGAACAAC TGGCAGGTGT 2041 TTCTGGATGC ATATCGGCGC TACTCAGGCC CGTGGTCCAA GATCTATTTT GTATTGTGGT 2101 GGCTGGTGTC GTCTGTCATC TGGGTCAACC TGTTTCTGGC CCTGATTCTG GAGAACTTCC 2161 TTCACAAGTG GGACCCCCGC AGCCACCTGC AGCCCCTTGC TGGGACCCCA GAGGCCACCT 2221 ACCAGATGAC TGTGGAGCTC CTGTTCAGGG ATATTCTGGA GGAGCCCGAG GAGGATGAGC 2281 TCACAGAGAG GCTGAGCCAG CACCCGCACC TGTGGCTGTG CAGGTTGCCA ACTTTCTTGT 2341 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2401 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2461 GAAAGGACGA TTAGTAACGG GGGTCCGAAA AGAAACGCGT TAAGTCgaca atcaacctct 2521 ggattacaaa atttgtgaaa gatt