Construct: ORF TRCN0000480857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006497.1_s317c1
- Derived from:
- ccsbBroadEn_07141
- DNA Barcode:
- GTGCCCTAGATTAAACAACGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YY1 (7528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480857
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7528 | YY1 | YY1 transcription factor | NM_003403.5 | 99.9% | 99.7% | 11G>A |
| 2 | mouse | 22632 | Yy1 | YY1 transcription factor | NM_009537.3 | 94.8% | 98.3% | (many diffs) |
| 3 | mouse | 22632 | Yy1 | YY1 transcription factor | XM_006515820.3 | 72% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcggacgac accctctaca tcgccacgga cggctcggag atgccggccg 121 agatcgtgga gctgcacgag atcgaggtgg agaccatccc ggtggagacc atcgagacca 181 cagtggtggg cgaggaggag gaggaggacg acgacgacga ggacggcggc ggtggcgacc 241 acggcggcgg gggcggccac gggcacgccg gccaccacca ccaccaccat caccaccacc 301 accacccgcc catgatcgct ctgcagccgc tggtcaccga cgacccgacc caggtgcacc 361 accaccagga ggtgatcctg gtgcagacgc gcgaggaggt ggtgggcggc gacgactcgg 421 acgggctgcg cgccgaggac ggcttcgagg atcagattct catcccggtg cccgcgccgg 481 ccggcggcga cgacgactac attgaacaaa cgctggtcac cgtggcggcg gccggcaaga 541 gcggcggcgg cggctcgtcg tcgtcgggag gcggccgcgt caagaagggc ggcggcaaga 601 agagcggcaa gaagagttac ctcagcggcg gggccggcgc ggcgggcggc ggcggcgccg 661 acccgggcaa caagaagtgg gagcagaagc aggtgcagat caagaccctg gagggcgagt 721 tctcggtcac catgtggtcc tcagatgaaa aaaaagatat tgaccatgag acagtggttg 781 aagaacagat cattggagag aactcacctc ctgattattc agaatatatg acaggaaaga 841 aacttccTCC TGGAGGAATA CCTGGCATTG ACCTCTCAGA TCCCAAACAA CTGGCAGAAT 901 TTGCTAGAAT GAAGCCAAGA AAAATTAAAG AAGATGATGC TCCAAGAACA ATAGCTTGCC 961 CTCATAAAGG CTGCACAAAG ATGTTCAGGG ATAACTCGGC CATGAGAAAA CATCTGCACA 1021 CCCACGGTCC CAGAGTCCAC GTCTGTGCAG AATGTGGCAA AGCTTTTGTT GAGAGTTCAA 1081 AACTAAAACG ACACCAACTG GTTCATACTG GAGAGAAGCC CTTTCAGTGC ACGTTCGAAG 1141 GCTGTGGGAA ACGCTTTTCA CTGGACTTCA ATTTGCGCAC ACATGTGCGA ATCCATACCG 1201 GAGACAGGCC CTATGTGTGC CCCTTCGATG GTTGTAATAA GAAGTTTGCT CAGTCAACTA 1261 ACCTGAAATC TCACATCTTA ACACATGCTA AGGCCAAAAA CAACCAGTGC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGAGTGCCCT AGATTAAACA ACGATTTACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt